Long-term effect of plastic film mulching and fertilization on bacterial communities in a brown soil revealed by high through-put sequencing

被引:76
作者
Farmer, John [1 ]
Zhang, Bin [1 ]
Jin, Xinxin [1 ]
Zhang, Peng [2 ]
Wang, Jingkuan [1 ]
机构
[1] Shenyang Agr Univ, Land & Environm Coll, Shenyang, Peoples R China
[2] Shenyang Agr Univ, Coll Hort, Shenyang, Peoples R China
基金
中国国家自然科学基金;
关键词
Long-term fertilization; plastic film mulching; bacterial community structure; high through-put sequencing; soil properties; MICROBIAL ACTIVITY; NORTHEAST CHINA; DIVERSITY; MANAGEMENT; QUALITY; FUNGAL; MANURE; PRODUCTIVITY; ABUNDANCE; DYNAMICS;
D O I
10.1080/03650340.2016.1193667
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The objective of this study was to access the effect of long-term fertilization and film mulching application on soil properties and bacterial community structure. We used 16S rRNA gene to investigate soil bacterial community composition by high through-put sequencing. The results demonstrated that predominant groups in the bacterial community were: Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria, Firmicutes, Planctomycetes, Gemmatimonadetes, Verrucomicrobia, Chloroflexi and Cyanobacteria. Longterm fertilization of combined manure and nitrogen application caused significant decrease in soil bacterial diversity and richness compared to non-fertilization control, though manure fertilization alone played a significant role in restoring bacterial diversity. Film mulching and manure fertilization significantly increased the relative abundances of soil bacterial groups mentioned above. Furthermore, film mulching played significant role in shaping the bacterial community structure regardless of fertilization. Redundancy analysis (RDA) showed that soil moisture content, pH, total N and soil organic C had significant (P < 0.05) influence on dominant bacterial groups. Altogether, plastic film mulching and manure application prevented loss of soil bacterial diversity and abundance during long-term fertilization. These findings showed the detrimental use of combined manure and nitrogen fertilization to soil microbes and the useful application of manure fertilization coupled with film mulching to soil biodiversity in long-term fertilization experiments.
引用
收藏
页码:230 / 241
页数:12
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