The antimicrobial mechanism of electrochemically activated water against Pseudomonas aeruginosa and Escherichia coli as determined by SDS-PAGE analysis

被引：51

作者：

Cloete, T. E. ^{[1
]}

Thantsha, M. S. ^{[1
]}

Maluleke, M. R. ^{[1
]}

Kirkpatrick, R. ^{[1
]}

机构：

[1] Univ Pretoria, Dept Plant Pathol & Microbiol, ZA-0001 Pretoria, South Africa

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2009年 / 107卷 / 02期

关键词：

anolyte; electrochemically activated water; Escherichia coli; Pseudomonas aeruginosa; SDS-PAGE; SUPER-OXIDIZED WATER; LISTERIA-MONOCYTOGENES; ELECTROLYZED WATER; STRESS; DISINFECTANTS; RESISTANCE; PROTEINS; BACTERIA; INACTIVATION; STERILOX(R);

D O I：

10.1111/j.1365-2672.2009.04233.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: To expose bacteria to anolyte and subsequently investigate the effect of anolyte on the protein profiles of treated bacteria. Methods and Results: Proteins were extracted from bacteria treated with different concentrations of anolyte and analysed using SDS-PAGE. Fewer and more faint protein bands were observed for concentrated halide anolyte treated bacteria when compared to untreated bacteria while extra protein bands were observed for bacteria exposed to dilute concentrations. Conclusions: The undiluted and the 10(-1) dilution of halide derived anolyte was effective in killing the test bacteria. Anolyte caused bacterial death by complete destruction of proteins or by causing oxidative stress which resulted in protein fragmentation. Significance and Impact of the Study: The results of this study provide information on the antimicrobial mechanism of anolyte on other bacteria for which the information is currently unavailable.

引用

页码：379 / 384

页数：6

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