Anti-inflammatory activity of soluble chito-oligosaccharides (CHOS) on VitD3-induced human THP-1 monocytes

被引：29

作者：

Jitprasertwong, Paiboon ^{[1
]}

Khamphio, Munthipha ^{[2
]}

Petsrichuang, Phornsiri ^{[2
]}

Eijsink, Vincent G. H. ^{[3
]}

Poolsri, Wanangkan ^{[4
]}

Muanprasat, Chatchai ^{[4
]}

Rangnoi, Kuntalee ^{[2
]}

Yamabhai, Montarop ^{[2
]}

机构：

[1] Suranaree Univ Technol SUT, Sch Geriatr Oral Hlth, Inst Dent, Nakhon Ratchasima, Thailand

[2] Suranaree Univ Technol SUT, Sch Biotechnol, Inst Agr Technol, Mol Biotechnol Lab, Nakhon Ratchasima, Thailand

[3] Norwegian Univ Life Sci NMBU, Fac Chem Biotechnol & Food Sci, As, Norway

[4] Mahidol Univ, Ramathibodi Hosp, Fac Med, Chakri Naruebodindra Med Inst, Samut Prakan, Thailand

来源：

PLOS ONE | 2021年 / 16卷 / 02期

关键词：

POTENTIAL APPLICATIONS; CHITOOLIGOSACCHARIDES; ACTIVATION; DIFFERENTIATION; DEGRADATION; CHITOSANASE; DAMAGE;

D O I：

10.1371/journal.pone.0246381

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chito-oligosaccharides (CHOS) are oligomers of D-glucosamine and N-acetyl-glucosamine. Anti-inflammatory activities of a wide variety of CHOS mixtures have previously been reported, mainly based on studies with mouse models and murine macrophages. Since the mouse and human immune systems are quite different, gaining insight into the activity of CHOS on human cell lines, using well-characterized CHOS mixtures, is of considerable interest. Bacillus subtilis chitosanase (BsCsn46A) can efficiently convert chitosan to mixtures of water soluble low molecular weight CHOS. Here, the anti-inflammatory activity of a properly characterized CHOS mixture was studied, using human THP-1 cells that were differentiated to mature monocytes using vitamin D3. Addition of CHOS reduced the production of multiple pro-inflammatory cytokines associated with bacterial lipopolyssacharide (LPS)-stimulated inflammation, in a dose-dependent manner and without affecting cell viability. Interestingly, only minimal effects of CHOS were observed in similar experiments with phorbol 12-myristate 13-acetate- (PMA-) differentiated, macrophage-like, THP-1 cells. Altogether, in addition to showing promising biological effects of well-characterized low molecular weight soluble CHOS in a human system, the present study also points at Vitamin D3-stimulated THP-1 cells as a favorable system for assessing the anti-inflammatory activity of bioactive compounds.

引用

页数：17