Clonal Expansion of Sulfonamide Resistant Escherichia coli Isolates Recovered from Diarrheic Calves

被引：0
|
作者：
Adnan, Muhammad [1 ]
Khan, Hamayun [2 ]
Kashif, Jam [1 ]
Ahmad, Shakoor [2 ]
Gohar, Ali [3 ]
Ali, Amjad [4 ]
Khan, Muhammad Atif [1 ]
Shah, Said Sajjad Ali [2 ]
Hassan, Mohammad Farooque [7 ]
Irshad, Muhammad [5 ]
Khan, Nazir Ahmad [6 ]
Rahman, Sadeeq Ur [3 ]
机构：
[1] Sindh Agr Univ Tando Jam, Dept Pharmacol, Tandojam, Pakistan
[2] Univ Agr Peshawar, Dept Anim Hlth, Peshawar, Pakistan
[3] Abdul Wali Khan Univ, Coll Vet Sci & AH, Mardan, Pakistan
[4] Hazara Univ, Ctr Human Genet, Mansehra, Pakistan
[5] Aljouf Univ, Dept Prevent Dent, Sakakah, Saudi Arabia
[6] Univ Agr, Dept Nutr, Peshawar, Pakistan
[7] Shaheed Benazir Bhutto Univ Vet & Anim Sci, Sakrand Sindh, Pakistan
来源：
PAKISTAN VETERINARY JOURNAL | 2017年 / 37卷 / 02期
关键词：
Diarrhea; E; coli; Minimum inhibitory concentration; Sul genes; Sulfadimidine resistance; GENES; SUL1;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A total of 30 Escherichia coli isolates from 50 random samples of diarrheic calves were screened against resistance to sulfonamides. Minimum inhibitory concentration (MIC) by microdilution method indicated that 25/30 (83.3%) were found resistant to sulfadimidine, 27/30 (90%) were resistant to sulfadiazine, while 22/30 (73.3%) were found resistant to sulfamethaxole. These phenotypically sulfonamide resistant isolates were then probed for the presence of Sul genes (Sul-1-3) by polymerase chain reaction (PCR) using specific primers. PCR results revealed that 23/25, 25/27 and 20/22 isolates carried Sul-1 gene, respectively. Interestingly, all these isolates were found negative for the presence of Sul-2 and Sul-3 genes. Of note, no Sul genes could be verified in 02 phenotypically resistant isolates. The abundance of Sul1 gene and absence of Sul-2 and Sul-3 genes indicating a clonal expansion of sulfonamide resistant E. coli that might be linked to excessive abuse of sulfonamides in animals. (C) 2017 PVJ. All rights reserved
引用
收藏
页码：230 / 232
页数：3
相关论文
共 50 条
  • [31] Resistome analysis of Escherichia coli isolates from layers in Hungary
    Kerek, Adam
    Szabo, Abel
    Banyai, Krisztian
    Kaszab, Eszter
    Bali, Krisztina
    Papp, Marton
    Kovacs, Laszlo
    Jerzsele, Akos
    ACTA VETERINARIA HUNGARICA, 2024, 72 (01) : 1 - 10
  • [32] Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil
    Salvadori, MR
    Valadares, GF
    Leite, DD
    Blanco, J
    Yano, T
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2003, 34 (03) : 230 - 235
  • [33] Proteomic analysis of multidrug resistant Escherichia coli strains from scouring calves
    Gautam, Ablesh
    Vinson, Heather M.
    Gibbs, Penelope S.
    Olet, Susan
    Barigye, Robert
    VETERINARY MICROBIOLOGY, 2011, 151 (3-4) : 363 - 371
  • [34] Antibiotic resistant shiga toxin producing Escherichia coli isolates from milk and milk products
    Sobeih, A. M. K.
    Moawad, A. A.
    Sharshar, T. A.
    JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, 2023, 74 (01): : 5283 - 5290
  • [35] Phenotypic and genotypic characterization of antibiotic-resistant in Escherichia coli isolates from patients with diarrhea
    Bonyadian, Mojtaba
    Barati, Sara
    Mahzounieh, Mohammad Reza
    IRANIAN JOURNAL OF MICROBIOLOGY, 2019, 11 (03) : 220 - 224
  • [36] High-Level Multidrug-Resistant Escherichia coli Isolates from Wild Birds in a Large Urban Environment
    Batalha de Jesus, Alice Aurora
    Rangel Freitas, Andrea Andrade
    de Souza, Juliana Carmo
    Martins, Natacha
    Batista Botelho, Larissa Alvarenga
    Carvalho Girao, Valeria Brigido
    Teixeira, Lucia Martins
    Riley, Lee Woodland
    Moreira, Beatriz Meurer
    MICROBIAL DRUG RESISTANCE, 2019, 25 (02) : 167 - 172
  • [37] Genotypic characterization of quinolone resistant-Escherichia coli isolates from retail food in Morocco
    Nayme, Kaotar
    Barguigua, Abouddihaj
    Bouchrif, Brahim
    Karraouan, Bouchra
    El Otmani, Fatima
    Elmdaghri, Naima
    Zerouali, Khalid
    Timinouni, Mohammed
    JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2017, 52 (02) : 107 - 114
  • [38] Virulence genotyping of Escherichia coli isolates from diarrheic and urinary tract infections in relation to phylogeny in southeast of Iran
    Alizade, H.
    Ghanbarpour, R.
    Aflatoonian, M. R.
    TROPICAL BIOMEDICINE, 2014, 31 (01) : 174 - 182
  • [39] The Clonal Distribution and Diversity of Extraintestinal Escherichia coli Isolates Vary According to Patient Characteristics
    Banerjee, Ritu
    Johnston, Brian
    Lohse, Christine
    Chattopadhyay, Sujay
    Tchesnokova, Veronika
    Sokurenko, Evgeni V.
    Johnson, James R.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (12) : 5912 - 5917
  • [40] Emergence of fluoroquinolone-resistant clonal group A: clonal analysis of Norwegian and Russian E. coli isolates
    Strand, Linda
    Jenkins, Andrew
    Grude, Nils
    Allum, Anne-Gry
    Mykland, Hilde-Christine
    Nowrouzian, Fourough L.
    Kristiansen, Bjorn-Erik
    APMIS, 2010, 118 (08) : 571 - 577
12345