Clonal Expansion of Sulfonamide Resistant Escherichia coli Isolates Recovered from Diarrheic Calves

被引：0
|
作者：
Adnan, Muhammad [1 ]
Khan, Hamayun [2 ]
Kashif, Jam [1 ]
Ahmad, Shakoor [2 ]
Gohar, Ali [3 ]
Ali, Amjad [4 ]
Khan, Muhammad Atif [1 ]
Shah, Said Sajjad Ali [2 ]
Hassan, Mohammad Farooque [7 ]
Irshad, Muhammad [5 ]
Khan, Nazir Ahmad [6 ]
Rahman, Sadeeq Ur [3 ]
机构：
[1] Sindh Agr Univ Tando Jam, Dept Pharmacol, Tandojam, Pakistan
[2] Univ Agr Peshawar, Dept Anim Hlth, Peshawar, Pakistan
[3] Abdul Wali Khan Univ, Coll Vet Sci & AH, Mardan, Pakistan
[4] Hazara Univ, Ctr Human Genet, Mansehra, Pakistan
[5] Aljouf Univ, Dept Prevent Dent, Sakakah, Saudi Arabia
[6] Univ Agr, Dept Nutr, Peshawar, Pakistan
[7] Shaheed Benazir Bhutto Univ Vet & Anim Sci, Sakrand Sindh, Pakistan
来源：
PAKISTAN VETERINARY JOURNAL | 2017年 / 37卷 / 02期
关键词：
Diarrhea; E; coli; Minimum inhibitory concentration; Sul genes; Sulfadimidine resistance; GENES; SUL1;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A total of 30 Escherichia coli isolates from 50 random samples of diarrheic calves were screened against resistance to sulfonamides. Minimum inhibitory concentration (MIC) by microdilution method indicated that 25/30 (83.3%) were found resistant to sulfadimidine, 27/30 (90%) were resistant to sulfadiazine, while 22/30 (73.3%) were found resistant to sulfamethaxole. These phenotypically sulfonamide resistant isolates were then probed for the presence of Sul genes (Sul-1-3) by polymerase chain reaction (PCR) using specific primers. PCR results revealed that 23/25, 25/27 and 20/22 isolates carried Sul-1 gene, respectively. Interestingly, all these isolates were found negative for the presence of Sul-2 and Sul-3 genes. Of note, no Sul genes could be verified in 02 phenotypically resistant isolates. The abundance of Sul1 gene and absence of Sul-2 and Sul-3 genes indicating a clonal expansion of sulfonamide resistant E. coli that might be linked to excessive abuse of sulfonamides in animals. (C) 2017 PVJ. All rights reserved
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页码：230 / 232
页数：3
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