Clonal Expansion of Sulfonamide Resistant Escherichia coli Isolates Recovered from Diarrheic Calves

被引：0
|
作者：
Adnan, Muhammad [1 ]
Khan, Hamayun [2 ]
Kashif, Jam [1 ]
Ahmad, Shakoor [2 ]
Gohar, Ali [3 ]
Ali, Amjad [4 ]
Khan, Muhammad Atif [1 ]
Shah, Said Sajjad Ali [2 ]
Hassan, Mohammad Farooque [7 ]
Irshad, Muhammad [5 ]
Khan, Nazir Ahmad [6 ]
Rahman, Sadeeq Ur [3 ]
机构：
[1] Sindh Agr Univ Tando Jam, Dept Pharmacol, Tandojam, Pakistan
[2] Univ Agr Peshawar, Dept Anim Hlth, Peshawar, Pakistan
[3] Abdul Wali Khan Univ, Coll Vet Sci & AH, Mardan, Pakistan
[4] Hazara Univ, Ctr Human Genet, Mansehra, Pakistan
[5] Aljouf Univ, Dept Prevent Dent, Sakakah, Saudi Arabia
[6] Univ Agr, Dept Nutr, Peshawar, Pakistan
[7] Shaheed Benazir Bhutto Univ Vet & Anim Sci, Sakrand Sindh, Pakistan
来源：
PAKISTAN VETERINARY JOURNAL | 2017年 / 37卷 / 02期
关键词：
Diarrhea; E; coli; Minimum inhibitory concentration; Sul genes; Sulfadimidine resistance; GENES; SUL1;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A total of 30 Escherichia coli isolates from 50 random samples of diarrheic calves were screened against resistance to sulfonamides. Minimum inhibitory concentration (MIC) by microdilution method indicated that 25/30 (83.3%) were found resistant to sulfadimidine, 27/30 (90%) were resistant to sulfadiazine, while 22/30 (73.3%) were found resistant to sulfamethaxole. These phenotypically sulfonamide resistant isolates were then probed for the presence of Sul genes (Sul-1-3) by polymerase chain reaction (PCR) using specific primers. PCR results revealed that 23/25, 25/27 and 20/22 isolates carried Sul-1 gene, respectively. Interestingly, all these isolates were found negative for the presence of Sul-2 and Sul-3 genes. Of note, no Sul genes could be verified in 02 phenotypically resistant isolates. The abundance of Sul1 gene and absence of Sul-2 and Sul-3 genes indicating a clonal expansion of sulfonamide resistant E. coli that might be linked to excessive abuse of sulfonamides in animals. (C) 2017 PVJ. All rights reserved
引用
收藏
页码：230 / 232
页数：3
相关论文
共 50 条
  • [1] High Biofilm-Forming Ability and Clonal Dissemination among Colistin-Resistant Escherichia coli Isolates Recovered from Cows with Mastitis, Diarrheic Calves, and Chickens with Colibacillosis in Tunisia
    Dhaouadi, Sana
    Romdhani, Amel
    Bouglita, Wafa
    Chedli, Salsabil
    Chaari, Soufiene
    Soufi, Leila
    Cherif, Ameur
    Mnif, Wissem
    Abbassi, Mohamed Salah
    Elandoulsi, Ramzi Boubaker
    LIFE-BASEL, 2023, 13 (02):
  • [2] Isolation of multidrug-resistant Escherichia coli and Salmonella spp. from sulfonamide-treated diarrheic calves
    Haque, Mohammad Amdadul
    Hossain, Muhammad Tofazzal
    Islam, Md. Shafiqul
    Islam, Md. Zahorul
    Islam, Purba
    Shaha, Sourendra Nath
    Sikder, Mahmudul Hasan
    Rafiq, Kazi
    VETERINARY WORLD, 2022, 15 (12) : 2870 - 2876
  • [3] Antibiotic Resistant and Biofilm-Associated Escherichia coli Isolates from Diarrheic and Healthy Dogs
    Karahutova, Livia
    Mandelik, Rene
    Bujnakova, Dobroslava
    MICROORGANISMS, 2021, 9 (06)
  • [4] Molecular characterization and antimicrobial profiling of Escherichia coli isolates from diarrheic calves
    Sharma, R. K.
    Taku, A. K.
    Malik, Andleeb
    Bhat, M. A.
    Javed, Rabyia
    Badroo, G. A.
    Kour, Amitoz
    INDIAN JOURNAL OF ANIMAL SCIENCES, 2017, 87 (12): : 1467 - 1471
  • [5] Antimicrobial determinants of multidrug resistant Escherichia coli serotypes isolated from diarrheic calves
    Abdeen, Eman E.
    Nayel, Mohammed A.
    Hannan, H.
    Elsify, Ahmed
    Salama, Akram A.
    Zaghawa, Ahmed A.
    Almuzaini, Abdulaziz M.
    Elbehiry, Ayman
    Mousa, Walid S.
    BIOSCIENCE RESEARCH, 2019, 16 : 53 - 61
  • [6] Virulence factors of Escherichia coli isolated from diarrheic calves
    Rigobelo, E. C.
    Gamez, H. J.
    Marin, J. M.
    Macedo, C.
    Ambrosin, J. A.
    Avila, F. A.
    ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2006, 58 (03) : 305 - 310
  • [7] Occurrence of Escherichia coli Pathotypes in Diarrheic Calves in a Low-Income Setting
    Chekole, Wagaw Sendeku
    Adamu, Haileeyesus
    Sternberg-Lewrein, Susanna
    Magnusson, Ulf
    Tessema, Tesfaye Sisay
    PATHOGENS, 2023, 12 (01):
  • [8] Virulotyping, Antimicrobial Resistance Profiling and DNA Fingerprinting of Escherichia coli Isolates from Neonatal Calves
    Athira, V.
    Nair, Sonu S.
    Thomas, Prasad
    Inbaraj, Sophia
    Kumar, M. Suman
    Akkara, Thanksy S.
    Chaudhuri, Pallab
    Sharun, Khan
    Kumar, Bablu
    Chaturvedi, V. K.
    Dandapat, Premanshu
    Abhishek
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2024,
  • [9] Detection of Class 1 and 2 Integrons, β-Lactamase Genes and Molecular Characterization of Sulfonamide Resistance in Escherichia coli Isolates Recovered from Poultry in China
    Kashif, Jam
    Buriro, Rehana
    Memon, Javed
    Yaqoob, Muhammad
    Soomro, Jamila
    Diao Dongxue
    Huang Jinhu
    Wang Liping
    PAKISTAN VETERINARY JOURNAL, 2013, 33 (03) : 321 - 324
  • [10] Inhibitory potential of natural plant extracts against Escherichia coli strain isolated from diarrheic camel calves
    Bessalah, Salma
    Khorchani, Touhami
    Hammadi, Mohamed
    Faraz, Asim
    Mustafa, Ayman Balla
    OPEN VETERINARY JOURNAL, 2023, 13 (09) : 1082 - 1090
12345