Deproteinization of gellan gum produced by Sphingomonas paucimobilis ATCC 31461

被引：45

作者：

Wang, Xia ^{[1
]}

Yuan, Yong ^{[1
]}

Wang, Kainai ^{[1
]}

Zhang, Dezhong ^{[1
]}

Yang, Zhengting ^{[1
]}

Xu, Ping ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

JOURNAL OF BIOTECHNOLOGY | 2007年 / 128卷 / 02期

关键词：

gellan gum; deproteinization; protease;

D O I：

10.1016/j.jbiotec.2006.09.019

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Deproteinization is a technical bottleneck in the purification of viscous water-soluble polysaccharides. The aim of this work is to provide an appropriate approach to deproteinize crude gellan gum. Several methods of deproteinization were investigated, including Sevag method, alkaline protease, papain and neutral protease. The results revealed that Sevag method had high deproteinization efficiency (87.9%), but it showed dissatisfactory recovery efficiency of gellan gum (28.6%), which made it less advisable in industrial applications. The deproteinization by alkaline protease was demonstrated in this work for the first time, indicating alkaline protease was preferred in the deproteinization of crude gellan gum with high polysaccharide recovery (89.3%) and high deproteinization efficiency (86.4%). (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：403 / 407

页数：5