KLF4, Slug and EMT in Head and Neck Squamous Cell Carcinoma

被引：25

作者：

Ingruber, Julia ^{[1
]}

Savic, Dragana ^{[2
,3
]}

Steinbichler, Teresa Bernadette ^{[1
]}

Sprung, Susanne ^{[4
]}

Fleischer, Felix ^{[1
,5
]}

Glueckert, Rudolf ^{[1
]}

Schweigl, Gabriele ^{[1
]}

Skvortsova, Ira-Ida ^{[2
,3
]}

Riechelmann, Herbert ^{[1
]}

Dudas, Jozsef ^{[1
]}

机构：

[1] Med Univ Innsbruck, Univ Hosp Tyrol, Dept Otorhinolaryngol & Head & Neck Surg, A-6020 Innsbruck, Austria

[2] Med Univ Innsbruck, Dept Therapeut Radiol & Oncol, Lab Expt & Translat Res Radiat Oncol, EXTRO Lab, A-6020 Innsbruck, Austria

[3] Tyrolean Canc Res Inst, A-6020 Innsbruck, Austria

[4] Med Univ Innsbruck, Dept Pathol, A-6020 Innsbruck, Austria

[5] Med Univ Innsbruck, Dept Restorat & Operat Dent, A-6020 Innsbruck, Austria

来源：

CELLS | 2021年 / 10卷 / 03期

基金：

奥地利科学基金会;

关键词：

TissueFaxs; TWIST; ZEB; SCC-25; FaDu; UPCI-SCC090; TO-MESENCHYMAL TRANSITION; GENE-EXPRESSION; HUMAN-PAPILLOMAVIRUS; BREAST-CANCER; TUMOR-CELL; TGF-BETA; SURVIVAL;

D O I：

10.3390/cells10030539

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Epithelial to mesenchymal transition (EMT) is clinically relevant in head and neck squamous cell carcinoma (HNSCC). We hypothesized that EMT-transcription factors (EMT-TFs) and an anti-EMT factor, Kruppel-like-factor-4 (KLF4) regulate EMT in HNSCC. Ten control mucosa and 37 HNSCC tissue samples and three HNSCC cell lines were included for investigation of EMT-TFs, KLF4 and vimentin at mRNA and protein levels. Slug gene expression was significantly higher, whereas, KLF4 gene expression was significantly lower in HNSCC than in normal mucosa. In the majority of HNSCC samples, there was a significant negative correlation between KLF4 and Slug gene expression. Slug gene expression was significantly higher in human papilloma virus (HPV) negative HNSCC, and in tumor samples with irregular p53 gene sequence. Transforming-growth-factor-beta-1 (TGF- beta 1) contributed to downregulation of KLF4 and upregulation of Slug. Two possible regulatory pathways could be suggested: (1) EMT-factors induced pathway, where TGF-beta 1 induced Slug together with vimentin, and KLF4 was down regulated at the same time; (2) p53 mutations contributed to upregulation and stabilization of Slug, where also KLF4 could co-exist with EMT-TFs.

引用

页码：1 / 23

页数：22

共 39 条

[1] Mutant p53 accumulation in human breast cancer is not an intrinsic property or dependent on structural or functional disruption but is regulated by exogenous stress and receptor status [J].