Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp ALSHL3

被引：14
|
作者：
Vidilaseris, Keni [1 ]
Hidayat, Karina [1 ]
Retnoningrum, Debbie S. [2 ,3 ]
Nurachman, Zeily [1 ]
Noer, Achmad Saefuddin [1 ]
Natalia, Dessy [1 ,3 ]
机构：
[1] Bandung Inst Technol, Div Biochem, Fac Math & Nat Sci, Bandung 40132, Indonesia
[2] Bandung Inst Technol, Sch Pharm, Bandung 40132, Indonesia
[3] Bandung Inst Technol, Ctr Life Sci, Bandung 40132, Indonesia
来源：
BIOLOGIA | 2009年 / 64卷 / 06期
关键词：
alpha-amylase; Bacillus filicolonicus; raw starch; maize; marine; BINDING DOMAIN; PURIFICATION; STRAIN; DEGRADATION; EVOLUTION; ENZYMES; FAMILY;
D O I：
10.2478/s11756-009-0190-8
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An Indonesian marine bacterial isolate, which belongs to genus of Bacillus sp. based on 16S rDNA analysis and was identified as Bacillus filicolonicus according to its morphology and physiology, produced a raw starch degrading alpha-amylase. The partially purified alpha-amylase using a maize starch affinity method exhibited an optimum pH and temperature of 6.0 and 60A degrees C, respectively. The enzyme retained 72% of its activity in the presence of 1.5 M NaCl. Scanning electron micrographs showed that the alpha-amylase was capable of degrading starch granules of rice and maize. This alpha-amylase from Bacillus sp. ALSHL3 was classified as a saccharifying enzyme since its major final degradation product was glucose, maltose, and maltotriose.
引用
收藏
页码：1047 / 1052
页数：6
相关论文
共 50 条
  • [1] Biochemical characterization of a raw starch degrading α-amylase from the Indonesian marine bacterium Bacillus sp. ALSHL3
    Keni Vidilaseris
    Karina Hidayat
    Debbie S. Retnoningrum
    Zeily Nurachman
    Achmad Saefuddin Noer
    Dessy Natalia
    Biologia, 2009, 64 : 1047 - 1052
  • [2] Isolation, expression, and characterization of raw starch degrading alpha-amylase from a marine lake Bacillus megaterium NL3
    Shofiyah, Sofi Siti
    Yuliani, Dewi
    Widya, Nurul
    Sarian, Fean D.
    Puspasari, Fernita
    Radjasa, Ocky Karna
    Ihsanawati
    Natalia, Dessy
    HELIYON, 2020, 6 (12)
  • [3] Identification and characterization of a novel raw-starch-degrading a-amylase (AmyASS) from the marine fish pathogen Aeromonas salmonicida ssp salmonicida
    Peng, Hui
    Chen, Maojiao
    Yi, Lu
    Zhang, Xiaohan
    Wang, Min
    Xiao, Yazhong
    Zhang, Nannan
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2015, 119 : 71 - 77
  • [4] Halotolerant, acid-alkali stable, chelator resistant and raw starch digesting α-amylase from a marine bacterium Bacillus subtilis S8-18
    Kalpana, Balu Jancy
    Pandian, Shunmugiah Karutha
    JOURNAL OF BASIC MICROBIOLOGY, 2014, 54 (08) : 802 - 811
  • [5] Raw starch-degrading α-amylase from Bacillus aquimaris MKSC 6.2: isolation and expression of the gene, bioinformatics and biochemical characterization of the recombinant enzyme
    Puspasari, F.
    Radjasa, O. K.
    Noer, A. S.
    Nurachman, Z.
    Syah, Y. M.
    van der Maarel, M.
    Dijkhuizen, L.
    Janecek, S.
    Natalia, D.
    JOURNAL OF APPLIED MICROBIOLOGY, 2013, 114 (01) : 108 - 120
  • [6] The raw starch-degrading alkaline amylase of Bacillus sp IMD 370
    Kelly, CT
    McTigue, MA
    Doyle, EM
    Fogarty, WM
    JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1995, 15 (05): : 446 - 448
  • [7] Characteristics of raw starch degrading α-amylase from Bacillus aquimaris MKSC 6.2 associated with soft coral Sinularia sp.
    Puspasari, Fernita
    Nurachman, Zeily
    Noer, Achmad Saefuddin
    Radjasa, Ocky Karna
    van der Maarel, Marc J. E. C.
    Natalia, Dessy
    STARCH-STARKE, 2011, 63 (08): : 461 - 467
  • [8] Purification and properties of the raw starch-degrading α-amylase of Bacillus sp. IMD 434
    Hamilton, LM
    Kelly, CT
    Fogarty, WM
    BIOTECHNOLOGY LETTERS, 1999, 21 (02) : 111 - 115
  • [9] Biochemical Characterization of Raw-starch-digesting Alpha Amylase Purified from Bacillus amyloliquefaciens
    Gangadharan, Dhanya
    Nampoothiri, K. Madhavan
    Sivaramakrishnan, Swetha
    Pandey, Ashok
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 158 (03) : 653 - 662
  • [10] Production of raw-starch-digesting α-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization
    Bozic, Natasa
    Slavic, Marinela Sokarda
    Gavrilovic, Anja
    Vujcic, Zoran
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2014, 37 (07) : 1353 - 1360
12345