A reevaluation of the contribution of NRT1.1 to nitrate uptake in Arabidopsis under low-nitrate supply

被引：28
|
作者：
Ye, Jia Yuan [1 ]
Tian, Wen Hao [1 ]
Jin, Chong Wei [1 ]
机构：
[1] Zhejiang Univ, Coll Nat Resources & Environm Sci, Minist Educ, Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Zhejiang, Peoples R China
来源：
FEBS LETTERS | 2019年 / 593卷 / 15期
基金：
中国国家自然科学基金;
关键词：
Arabidopsis thaliana; dual-affinity nitrate transporter; nitrate uptake; NRT1; 1; NRT2; 2; CADMIUM UPTAKE; CHL1-5; MUTANT; UPTAKE SYSTEM; TRANSPORTER; THALIANA; ROOTS; PLAYS; AVAILABILITY; ACQUISITION; EXPRESSION;
D O I：
10.1002/1873-3468.13473
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NRT1.1 has been previously characterized as a dual-affinity nitrate transporter in Arabidopsis, though several lines of evidence have raised questions regarding its high-affinity function in nitrate uptake. Here, we show that the induction of NRT2.1- and NRT2.2-mediated nitrate uptake interferes with measurements of the contribution of NRT1.1 to high-affinity uptake using nrt1.1 mutants. Therefore, a nrt1.1/2.1/2.2 triple mutant was generated to reevaluate the role of NRT1.1 in high-affinity nitrate uptake. This triple mutant has a lower rate of nitrate uptake than the nrt2.1/2.2 double mutant under low external nitrate supply, resulting in a lower growth rate than that of the double mutant. Therefore, we conclude that NRT1.1-mediated high-affinity nitrate uptake is necessary for plant growth under low-nitrate conditions.
引用
收藏
页码：2051 / 2059
页数：9
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