Effects of local structural transformation of lipid-like compounds on delivery of messenger RNA

被引：33
|
作者：
Li, Bin [1 ]
Luo, Xiao [1 ]
Deng, Binbin [2 ]
Giancola, JoLynn B. [3 ]
McComb, David W. [2 ]
Schmittgen, Thomas D. [4 ]
Dong, Yizhou [1 ]
机构：
[1] Ohio State Univ, Coll Pharm, Div Pharmaceut & Pharmaceut Chem, 500 W 12Th Ave, Columbus, OH 43210 USA
[2] Ohio State Univ, Ctr Electron Microscopy & Anal, Dept Mat Sci & Engn, Columbus, OH 43212 USA
[3] Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 USA
[4] Univ Florida, Coll Pharm, Div Pharmaceut, Gainesville, FL 32610 USA
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
关键词：
IN-VIVO; COMBINATORIAL LIBRARY; NANOPARTICLES; NANOMATERIALS; OPTIMIZATION; FORMULATIONS; EXPRESSION; CELLS;
D O I：
10.1038/srep22137
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Lipid-like nanoparticles (LLNs) have shown great potential for RNA delivery. Lipid-like compounds are key components in LLNs. In this study, we investigated the effects of local structural transformation of lipid-like compounds on delivery of messenger RNA. Our results showed that position change of functional groups on lipid-like compounds can dramatically improve delivery efficiency. We then optimized formulation ratios of TNT-b(10) LLNs, a lead material, increasing delivery efficiency over 2-fold. More importantly, pegylated TNT-b(10) LLNs is stable for over four weeks and is over 10-fold more efficient than that of its counterpart TNT-a(10) LLNs. Additionally, the optimal formulation O-TNT-b(10) LLNs is capable of delivering mRNA encoding luciferase in vivo. These results provide useful insights into the design of next generation LLNs for mRNA delivery.
引用
收藏
页数：8
相关论文
共 50 条
  • [1] Effects of local structural transformation of lipid-like compounds on delivery of messenger RNA
    Bin Li
    Xiao Luo
    Binbin Deng
    JoLynn B. Giancola
    David W. McComb
    Thomas D. Schmittgen
    Yizhou Dong
    Scientific Reports, 6
  • [2] Lipid-Like Nanoparticles for Small Interfering RNA Delivery to Endothelial Cells
    Cho, Seung-Woo
    Goldberg, Michael
    Son, Sun Mi
    Xu, Qiaobing
    Yang, Fan
    Mei, Ying
    Bogatyrev, Said
    Langer, Robert
    Anderson, Daniel G.
    ADVANCED FUNCTIONAL MATERIALS, 2009, 19 (19) : 3112 - 3118
  • [3] Self-Degradable Lipid-Like Materials Based on "Hydrolysis accelerated by the intra-Particle Enrichment of Reactant (HyPER)" for Messenger RNA Delivery
    Tanaka, Hiroki
    Takahashi, Tatsunari
    Konishi, Manami
    Takata, Nae
    Gomi, Masaki
    Shirane, Daiki
    Miyama, Ryo
    Hagiwara, Shinya
    Yamasaki, Yuki
    Sakurai, Yu
    Ueda, Keisuke
    Higashi, Kenjirou
    Moribe, Kunikazu
    Shinsho, Eiji
    Nishida, Ruka
    Fukuzawa, Kaori
    Yonemochi, Etsuo
    Okuwaki, Koji
    Mochizuki, Yuji
    Nakai, Yuta
    Tange, Kota
    Yoshioka, Hiroki
    Tamagawa, Shinya
    Akita, Hidetaka
    ADVANCED FUNCTIONAL MATERIALS, 2020, 30 (34)
  • [4] A combinatorial library of lipid-like materials for delivery of RNAi therapeutics
    Akinc, Akin
    Zumbuehl, Andreas
    Goldberg, Michael
    Leshchiner, Elizaveta S.
    Busini, Valentina
    Hossain, Naushad
    Bacallado, Sergio A.
    Nguyen, David N.
    Fuller, Jason
    Alvarez, Rene
    Borodovsky, Anna
    Borland, Todd
    Constien, Rainer
    de Fougerolles, Antonin
    Dorkin, J. Robert
    Jayaprakash, K. Narayanannair
    Jayaraman, Muthusamy
    John, Matthias
    Koteliansky, Victor
    Manoharan, Muthiah
    Nechev, Lubomir
    Qin, June
    Racie, Timothy
    Raitcheva, Denitza
    Rajeev, Kallanthottathil G.
    Sah, Dinah W. Y.
    Soutschek, Juergen
    Toudjarska, Ivanka
    Vornlocher, Hans-Peter
    Zimmermann, Tracy S.
    Langer, Robert
    Anderson, Daniel G.
    NATURE BIOTECHNOLOGY, 2008, 26 (05) : 561 - 569
  • [5] Design Principles of Lipid-like Ionic Liquids for Gene Delivery
    Siegel, David J.
    Anderson, Grace, I
    Paul, Lauren M.
    Seibert, Philipp J.
    Hillesheim, Patrick C.
    Sheng, Yinghong
    Zeller, Matthias
    Taubert, Andreas
    Werner, Peter
    Balischewski, Christian
    Michael, Scott F.
    Mirjafari, Arsalan
    ACS APPLIED BIO MATERIALS, 2021, 4 (06) : 4737 - 4743
  • [6] Targeted Drug Delivery in Lipid-like Nanocages and Extracellular Vesicles
    Sokolov, A. V.
    Kostin, N. N.
    Ovchinnikova, L. A.
    Lomakin, Y. A.
    Kudriaeva, A. A.
    ACTA NATURAE, 2019, 11 (02): : 28 - 41
  • [7] A combinatorial library of lipid-like materials for delivery of RNAi therapeutics
    Akin Akinc
    Andreas Zumbuehl
    Michael Goldberg
    Elizaveta S Leshchiner
    Valentina Busini
    Naushad Hossain
    Sergio A Bacallado
    David N Nguyen
    Jason Fuller
    Rene Alvarez
    Anna Borodovsky
    Todd Borland
    Rainer Constien
    Antonin de Fougerolles
    J Robert Dorkin
    K Narayanannair Jayaprakash
    Muthusamy Jayaraman
    Matthias John
    Victor Koteliansky
    Muthiah Manoharan
    Lubomir Nechev
    June Qin
    Timothy Racie
    Denitza Raitcheva
    Kallanthottathil G Rajeev
    Dinah W Y Sah
    Jürgen Soutschek
    Ivanka Toudjarska
    Hans-Peter Vornlocher
    Tracy S Zimmermann
    Robert Langer
    Daniel G Anderson
    Nature Biotechnology, 2008, 26 : 561 - 569
  • [8] Lipid-like Self-Assembling Peptide Nanovesicles for Drug Delivery
    Fatouros, Dimitrios G.
    Lamprou, Dimitrios A.
    Urquhart, Andrew J.
    Yannopoulos, Spyros N.
    Vizirianakis, Ioannis S.
    Zhang, Shuguang
    Koutsopoulos, Sotirios
    ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (11) : 8184 - 8189
  • [9] An Orthogonal Array Optimization of Lipid-like Nanoparticles for mRNA Delivery in Vivo
    Li, Bin
    Luo, Xiao
    Deng, Binbin
    Wang, Junfeng
    McComb, David W.
    Shi, Yimin
    Gaensler, Karin M. L.
    Tan, Xu
    Dunn, Amy L.
    Kerlin, Bryce A.
    Dong, Yizhou
    NANO LETTERS, 2015, 15 (12) : 8099 - 8107
  • [10] Enhanced Intracellular siRNA Delivery using Bioreducible Lipid-Like Nanoparticles
    Wang, Ming
    Alberti, Kyle
    Varone, Antonio
    Pouli, Dimitria
    Georgakoudi, Irene
    Xu, Qiaobing
    ADVANCED HEALTHCARE MATERIALS, 2014, 3 (09) : 1398 - 1403
12345