Uniformity and Sensitivity Improvements in Comprehensive Two-Dimensional Gas Chromatography Using Flame Ionization Detection with Post-Column Reaction

被引：9

作者：

Luong, Jim ^{[1
,2
]}

Hua, Yujuan ^{[1
]}

Gras, Ronda ^{[1
,2
]}

Shellie, Robert A. ^{[3
]}

机构：

[1] Dow Chem Canada ULC, Highway 15, Ft Saskatchewan, AB T8L 2P4, Canada

[2] Univ Tasmania, Australian Ctr Res Separat Sci ACROSS, Private Bag 75, Hobart, Tas 7001, Australia

[3] Deakin Univ, Sch Exercise & Nutr Sci, CASS, Burwood Highway, Burwood, Vic 3125, Australia

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 17期

关键词：

GC X GC; RESPONSE FACTORS; SULFUR; OIL;

D O I：

10.1021/acs.analchem.9b02159

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A 3D-printed microreactor for post-column reactions was successfully integrated with comprehensive two-dimensional gas chromatography. A two-stage post-column reaction provided a carbon-independent response, enhanced the flame ionization detection uniformity, and improved the detector sensitivity. These enhancements are critical to overcome challenges in analyses using comprehensive two-dimensional gas chromatography and flame ionization detection, which aim to separate and quantify multiple components. Post-column reaction flame ionization detection eliminated the requirement of multilevel and multicompound calibration, it enabled the determination of target analytes with a single-carbon-containing calibration compound with an accuracy of +/- 10%, and it improved the sensitivity for compounds that were not efficiently ionized by flame ionization detection. Extra column band-broadening caused by the incorporation of the 3D-printed microreactor was minimized using optimized reactor operating parameters and intercolumn connectivity. Chromatographic fidelity was in the practical domain of comprehensive 2D gas chromatography. Typical peak widths at half-height using the described approach ranged from 165 to 235 ms for probe compounds with retention factors spanning 5 < k < 40.

引用

页码：11223 / 11230

页数：8