Silver Nanoparticles-silsesquioxane Nanomaterial Applied to the Determination of 4-nitrophenol as a Biomarker

被引：22

作者：

Crocomo, Paola Zimmermann ^{[1
]}

Winiarski, Joao Paulo ^{[1
]}

de Barros, Marilia Reginato ^{[1
]}

Latocheski, Eloah ^{[1
]}

Nagurniak, Glaucio Regis ^{[2
]}

Tame Parreira, Renato Luis ^{[3
]}

Siebert, Diogo Alexandre ^{[1
]}

Micke, Gustavo Amadeu ^{[1
]}

Magosso, Herica Aparecida ^{[1
]}

Jost, Cristiane Luisa ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Dept Quim, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Pelotas, Dept Fis, POB 354, Pelotas, RS, Brazil

[3] Univ Franca, Nude Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP, Brazil

来源：

ELECTROANALYSIS | 2019年 / 31卷 / 12期

关键词：

hybrid material; silver nanoparticles electrochemical sensor; 4-nitrophenol; quantum chemical calculations; biomarker; GLASSY-CARBON ELECTRODE; SMALL-ANGLE SCATTERING; URINARY P-NITROPHENOL; CATALYTIC-REDUCTION; GREEN SYNTHESIS; ELECTROCHEMICAL DETERMINATION; PESTICIDES; GOLD; OXIDE; NANOCOMPOSITE;

D O I：

10.1002/elan.201900217

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel silsesquioxane material was synthetized and used as a stabilizing agent for silver nanoparticles. This hybrid material was characterized by FTIR, Si-29 CP-MAS NMR, C-13 DEPT 135 degrees NMR and TGA techniques and the silver nanoparticles were characterized from DLS, UV-Vis spectroscopy, zeta-potential, TEM and SAXS results. The silver nanoparticles obtained were spherical in shape with a diameter of 3.74 nm. The nanomaterial was successfully applied in the modification of a glassy carbon electrode and a pronounced current response was obtained in the determination of the biomarker 4-nitrophenol. Quantum chemical calculations, using density functional theory, were also performed in order to evaluate the redox properties of the analyte. Two different linear ranges were obtained applying optimal square wave voltammetry conditions. The reduction peak currents obtained were linear for 4-NP concentrations in the ranges of 0.29 to 1.50 mu mol L-1 (E-d=-0.6 V and t(d)=20 s) and 2.75 to 31.5 mu mol L-1, with a theoretical (signal to noise=3) limit of detection of 0.05 mu mol L-1 (t(d)=20 s). The proposed method was successfully applied to the determination of 4-NP in synthetic serum samples at different levels of 4-NP with a recovery range of 94-101 %. Validation was performed using a comparative method through the capillary electrophoresis (CE) technique.

引用

页码：2319 / 2329

页数：11