Cell Placement and Guidance on Substrates for Neurochip Interfaces

被引：13

作者：

Charrier, Anne ^{[1
,2
]}

Martinez, Dolores ^{[3
]}

Monette, Robert ^{[4
]}

Comas, Tanya ^{[4
]}

Movileanu, Raluca ^{[3
]}

Py, Christophe ^{[3
]}

Denhoff, Mike ^{[3
]}

Krantis, Anthony ^{[5
]}

Mealing, Geoff ^{[4
]}

机构：

[1] CNRS, Ctr Interdisciplinaire Nanosci Marseille, F-13288 Marseille 9, France

[2] CNR, Steacie Inst Mol Sci, Ottawa, ON, Canada

[3] CNR, Inst Microstruct Sci, Ottawa, ON, Canada

[4] CNR, Inst Biol Sci, Ottawa, ON, Canada

[5] Univ Ottawa, Ottawa, ON, Canada

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2010年 / 105卷 / 02期

关键词：

neurochip interface; cell placement; neurites guidance; proteins; patterning; PDMS stamp; NEURONAL NETWORKS; CLAMP; ELECTRODES; ARRAY; CONNECTIVITY; PROTEINS; PATTERNS; SURFACES;

D O I：

10.1002/bit.22539

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Interface devices such as integrated planar patch-clamp chips are being developed to study the electro-physiological activity of neuronal networks grown in vitro. The utility of such devices will be dependent upon the ability to align neurons with interface features on the chip by controlling neuronal placement and by guiding cell connectivity. In this paper, we present a strategy to accomplish this goal. Patterned chemical modification of SiN surfaces with poly-D-lysine transferred from PDMS stamps was used to promote adhesion and guidance of cryo-preserved primary rat cortical neurons. We demonstrate that these neurons can be positioned and grown over microhole features which will ultimately serve as patch-clamp interfaces on the chip. Biotechnol. Bioeng. 2010;105: 368-373. (C) 2009 Wiley Periodicals, Inc.

引用

页码：368 / 373

页数：6

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