A novel biosensor for the detection of zearalenone family mycotoxins in milk

被引：32

作者：

Valimaa, Anna-Liisa ^{[1
]}

Kivisto, Anniina T. ^{[1
]}

Leskinen, Piia I. ^{[3
]}

Karp, Matti T. ^{[1
,2
]}

机构：

[1] Tampere Univ Technol, Dept Chem & Bioengn, FI-33101 Tampere, Finland

[2] BCC, Tampere 33101, Finland

[3] Univ Turku, Dept Biol, FI-20014 Turku, Finland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 80卷 / 01期

关键词：

Zearalenone; Photinus pyralis; Luciferase reporter gene; Endocrine disrupter; BIOLUMINESCENT ASSAY; GAS-CHROMATOGRAPHY; BOVINE-MILK; DAIRY-COWS; CARRY-OVER; YEAST; METABOLISM; PRODUCTS; TRICHOTHECENES; ESTROGENICITY;

D O I：

10.1016/j.mimet.2009.10.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, a method for detecting estrogenic mycotoxin residues in milk was developed utilizing bioluminescent whole-cell biosensors. Milk products of various compositions were spiked with the estrogenic mycotoxins zearalenone and its metabolites zearalanone, alpha-zearalanol, beta-zearalanol, alpha-zearalenol and beta-zearalenol. The estrogenic response was detected by a whole-cell biosensor based on a genetically modified Saccharomyces cerevisiae strain that in the presence of an estrogenic compound produces firefly luciferase-enzyme and further light emission within a system provided with D-luciferin substrate. The results show that the yeast sensor reacts to mycotoxins with typical sigmoidal response at nanomolar concentrations. The response differs in different milk products with regard to the fat content of the milk. Due to short assay time of less than 3 h and automation the approach can be used as a bioavailability and activity screening method prior to more detailed chemical analysis. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：44 / 48

页数：5

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