Simultaneous determination of five major biologically active ingredients in different parts of Gardenia jasminoides fruits by HPLC with diode-array detection

被引：45

作者：

He, Mei-Lian ^{[1
]}

Cheng, Xiao-Wei ^{[1
]}

Chen, Jia-Kuan ^{[1
]}

Zhou, Tong-Shui ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Res Ctr Nat Prod, Shanghai 200433, Peoples R China

来源：

CHROMATOGRAPHIA | 2006年 / 64卷 / 11-12期

关键词：

column liquid chromatography; diode-array detection; geniposide and geniposidic acid; crocin; 1; and; 2; chlorogenic acid; Gardenia jasminoides;

D O I：

10.1365/s10337-006-0099-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive and specific HPLC method for the simultaneous separation and determination of geniposide, geniposidic acid, chlorogenic acid, crocin 1 and 2 in fruits of Gardenia jasminoides Ellis was developed. Optimum chromatographic performance was obtained with a C-18 column and acetonitrile-0.1% aqueous trifluoroacetic acid (v/v) as mobile phase. Isolated peaks were detected at 2,40 nm for geniposidic acid and geniposide, 330 nm for chlorogenic acid and 440 nm for crocin 1 and 2, respectively. Comparison of spectra recorded with a diode-array detector during elution of peaks enabled determination of method specificity. Quantitative determinations on different parts of gardenia fruit demonstrated that all these compounds were abundant mainly in pericarps and pulps and only iridoid glycosides were also presented in sepals and seeds of the fruits.

引用

页码：713 / 717

页数：5

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