Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

被引：49

作者：

Dajnoki, Zsolt ^{[1
,2
]}

Beke, Gabriella ^{[1
,2
]}

Kapitany, Aniko ^{[1
,2
]}

Mocsai, Gabor ^{[1
,2
]}

Gaspar, Krisztian ^{[1
,2
]}

Ruhl, Ralph ^{[3
,4
]}

Hendrik, Zoltan ^{[5
]}

Juhasz, Istvan ^{[2
]}

Zouboulis, Christos C. ^{[6
,7
,8
]}

Bacsi, Attila ^{[9
]}

Biro, Tamas ^{[9
,10
,11
]}

Torocsik, Daniel ^{[2
]}

Szegedi, Andrea ^{[1
,2
]}

机构：

[1] Univ Debrecen, Fac Med, Dept Dermatol, Div Dermatol Allergol, Nagyerdei St 98, H-4032 Debrecen, Hungary

[2] Univ Debrecen, Dept Dermatol, Fac Med, Debrecen, Hungary

[3] Univ Debrecen, MTA DE Publ Hlth Res Grp, Hungarian Acad Sci, Fac Publ Hlth, Debrecen, Hungary

[4] Paprika Bioanalyt Bt, Debrecen, Hungary

[5] Univ Debrecen, Dept Pathol, Fac Med, Debrecen, Hungary

[6] Dessau Med Ctr, Dept Dermatol, Dessau, Germany

[7] Dessau Med Ctr, Dept Venereol, Dessau, Germany

[8] Dessau Med Ctr, Dept Allergol & Immunol, Dessau, Germany

[9] Univ Debrecen, Dept Immunol, Fac Med, Debrecen, Hungary

[10] Univ Debrecen, Dept Physiol, Fac Med, Debrecen, Hungary

[11] Univ Debrecen, Fac Med, DE MTA Lendulet Cellular Physiol Res Grp, Debrecen, Hungary

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 2017年 / 137卷 / 05期

关键词：

THYMIC STROMAL LYMPHOPOIETIN; EPITHELIAL-CELLS; ATOPIC-DERMATITIS; DENDRITIC CELLS; T-CELLS; INFLAMMATION; DISEASE; BETA; KERATINOCYTES; HOMEOSTASIS;

D O I：

10.1016/j.jid.2016.12.025

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The microbial community exhibits remarkable diversity on topographically distinct skin regions, which may be accompanied by differences in skin immune characteristics. Our aim was to compare the immune milieu of healthy sebaceous gland-rich (SGR) and sebaceous gland-poor skin areas, and to analyze its changes in an inflammatory disease of SGR skin. For this purpose, immunohistochemical, immunocytochemical, and quantitative real-time PCR analyses of thymic stromal lymphopoietin (TSLP) and other cytokines, phenotypic immune cell markers and transcription factors were carried out in samples from sebaceous gland-poor, SGR skin and from papulopustular rosacea. TSLP mRNA and protein production was also studied in cultured keratinocytes. In SGR skin, higher TSLP expression, dendritic cell appearance without prominent activation, and T cell presence with IL-17/IL-10 cytokine milieu were detected compared with sebaceous gland-poor skin. Linoleic acid, a major sebum component, was found to induce TSLP expression dose-dependently in keratinocytes. In papulopustular rosacea, significantly decreased TSLP level and influx of inflammatory dendritic cells and T cells with IL-17/interferon-gamma cytokine milieu were observed. According to our results, SGR skin is characterized by a distinct, noninflammatory immune surveillance, which may explain the preferred localization of inflammatory skin diseases, and can influence future barrier repair therapeutic concepts.

引用

页码：1114 / 1125

页数：12

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