Impairment of endothelium-dependent relaxation of rabbit aortas by cigarette smoke extract - Role of free radicals and attenuation by captopril

被引：139

作者：

Ota, Y ^{[1
]}

Kugiyama, K ^{[1
]}

Sugiyama, S ^{[1
]}

Ohgushi, M ^{[1
]}

Matsumura, T ^{[1
]}

Doi, H ^{[1
]}

Ogata, N ^{[1
]}

Oka, H ^{[1
]}

Yasue, H ^{[1
]}

机构：

[1] KUMAMOTO UNIV, SCH MED, DIV CARDIOL, KUMAMOTO 860, JAPAN

来源：

ATHEROSCLEROSIS | 1997年 / 131卷 / 02期

关键词：

smoking; free radical scavengers; nitric oxide; endothelium; angiotensin converting enzyme inhibitors;

D O I：

10.1016/S0021-9150(97)06106-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of this study was to examine the effects of the water soluble component of cigarette smoke extract (CSE) on endothelium-dependent relaxation (EDR) of isolated rabbit aortas. The incubation with CSE was found to inhibit EDR in a dose-dependent manner. Go-incubation of the aortic strips with superoxide dismutase (SOD), N-acetylcysteine, glutathione or dimetyl sulfoxide (DMSO), free radical scavengers, attenuated the CSE-induced inhibition of the arterial relaxation. Co-incubation of the strips with captopril (3 mM), an angiotensin converting enzyme inhibitor, also attenuated CSE-induced impairment of vasorelaxation. In parallel experiments using cultured human endothelial cells, CSE suppressed endothelial release of NOx, stable metabolites of nitric oxide (NO). SOD, DMSO and captopril attenuated the suppression of NO production by CSE in association with reduction of free radicals, superoxide anions and hydroxyl radicals, in CSE solution. Neither lactate dehydrogenase release from the cultured endothelial cells nor cell death estimated by trypan blue exclusion test was found after the incubation of the cultured endothelial cells with CSE. The results indicate that free radicals in CSE induce the impairment of EDR, which may be partly due to suppression of NO production and is not due to non specific cytotoxicity by CSE. Captopril attenuates CSE-induced endothelial dysfunction partly through scavenging free radicals. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：195 / 202

页数：8

共 35 条

[1] EFFECT OF SMOKING ON THE ACTIVITY OF ISCHEMIC HEART-DISEASE [J].

BARRY, J ;

MEAD, K ;

NABEL, EG ;

ROCCO, MB ;

CAMPBELL, S ;

FENTON, T ;

MUDGE, GH ;

SELWYN, AP .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1989, 261 (03) :398-402

[2] Regression or progression - Dependency on vascular nitric oxide [J].

Candipan, RC ;

Wang, BY ;

Buitrago, R ;

Tsao, PS ;

Cooke, JP .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1996, 16 (01) :44-50

[3] CIGARETTE-SMOKING IS ASSOCIATED WITH DOSE-RELATED AND POTENTIALLY REVERSIBLE IMPAIRMENT OF ENDOTHELIUM-DEPENDENT DILATION IN HEALTHY-YOUNG ADULTS [J].

CELERMAJER, DS ;

SORENSEN, KE ;

GEORGAKOPOULOS, D ;

BULL, C ;

THOMAS, O ;

ROBINSON, J ;

DEANFIELD, JE .

CIRCULATION, 1993, 88 (05) :2149-2155

[4]

CHOW CK, 1986, J AM COLL NUTR, V5, P305

[5] FREE-RADICAL CHEMISTRY OF CIGARETTE-SMOKE AND ITS TOXICOLOGICAL IMPLICATIONS [J].

CHURCH, DF ;

PRYOR, WA .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1985, 64 :111-126

[6] EFFECTS OF ANGIOTENSIN CONVERTING ENZYME-INHIBITORS AND OF HYDRALAZINE ON ENDOTHELIAL FUNCTION IN HYPERTENSIVE RATS [J].

CLOZEL, M ;

KUHN, H ;

HEFTI, F .

HYPERTENSION, 1990, 16 (05) :532-540

[7]

Duthie G G, 1989, Nutr Res Rev, V2, P51, DOI 10.1079/NRR19890007

[8] UNDERSTANDING THE CONTROVERSY OVER THE IDENTITY OF EDRF [J].

FEELISCH, M ;

POEL, MT ;

ZAMORA, R ;

DEUSSEN, A ;

MONCADA, S .

NATURE, 1994, 368 (6466) :62-65

[9]

FREEMAN BA, 1982, LAB INVEST, V47, P412

[10]

GOLDSCHMIDT JE, 1991, J PHARMACOL EXP THER, V257, P1136

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