Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis
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作者:
Canakci, Cenk Fatih
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Ataturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Canakci, Cenk Fatih
[1
]
Canakci, Varol
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Ataturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Canakci, Varol
[1
]
Tatar, Abdulgani
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Ataturk Univ, Dept Genet, Fac Med, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Tatar, Abdulgani
[2
]
Eltas, Abubekir
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Ataturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Eltas, Abubekir
[1
]
Sezer, Ufuk
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Karadeniz Tech Univ, Dept Periodontol, Fac Dent, Trabzon, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Sezer, Ufuk
[3
]
Cicek, Yasin
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Ataturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Cicek, Yasin
[1
]
Oztas, Sitki
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Ataturk Univ, Dept Genet, Fac Med, TR-25240 Erzurum, TurkeyAtaturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
Oztas, Sitki
[2
]
机构:
[1] Ataturk Univ, Dept Periodontol, Fac Dent, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Dept Genet, Fac Med, TR-25240 Erzurum, Turkey
[3] Karadeniz Tech Univ, Dept Periodontol, Fac Dent, Trabzon, Turkey
periodontitis;
gingiva;
saliva;
8-hydroxyguanosine;
mitochondrial DNA deletion;
oxidative DNA damage;
REACTIVE OXYGEN;
DELETION;
STRESS;
MUTATIONS;
8-HYDROXY-2'-DEOXYGUANOSINE;
ACCUMULATION;
PROSTATE;
BLADDER;
D O I:
10.1007/s00005-009-0026-9
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
Introduction: Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG levels in saliva and mtDNA deletions in gingival tissue of patients with chronic periodontitis (CP) were evaluated. Materials and Methods: Gingival tissue and whole saliva samples were collected from 32 patients with CP and 32 healthy control subjects. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level (CAL), and probing depth (PD) were measured. Using the ELISA and polymerase chain reaction methods, the salivary 8-OHdG levels and the 7.4-kbp and 5-kbp mtDNA deletions were examined. Results: The 5-kbp mtDNA deletion was detected in 20 of the 32 periodontitis patients (62.5%), but was not detected in the healthy controls. The mean value of 8-OHdG in the saliva of the periodontitis patients with deleted mtDNA was significantly higher than in the patients with non-deleted mtDNA (p<0.01). Also, significant correlation was found between the occurrence of the 5-kbp mtDNA deletion and salivary 8-OHdG levels (p<0.01). Similar correlations were detected between salivary 8-OHdG levels and age, PD, and CAL (p<0.01, p<0.05). Conclusion: Increased oxidative stress may lead to premature oxidative DNA damage in the gingival tissue of periodontitis patients and the salivary 8-OHdG level may signify premature oxidative mtDNA damage in diseased gingival tissue.
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页码:205 / 211
页数:7
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Kakimoto, M
Inoguchi, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Inoguchi, T
Sonta, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Sonta, T
Yu, HY
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Yu, HY
Imamura, M
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Imamura, M
Etoh, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Etoh, T
Hashimoto, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Hashimoto, T
Nawata, H
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Kakimoto, M
Inoguchi, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Inoguchi, T
Sonta, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Sonta, T
Yu, HY
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Yu, HY
Imamura, M
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Imamura, M
Etoh, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Etoh, T
Hashimoto, T
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan
Hashimoto, T
Nawata, H
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Kyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, JapanKyushu Univ, Grad Sch Med Sci, Dept med & Bioregulat Sci, Fukuoka 8128582, Japan