Real-time monitoring for bioaerosols - flow cytometry

被引：17

作者：

Chen, Pei-Shih

Li, Chih-Shan ^{[1
]}

机构：

[1] Kaohsiung Med Univ, Dept Publ Hlth, Kaohsiung, Taiwan

[2] Natl Taiwan Univ, Coll Publ Hlth, Grad Inst Environm Hlth, Taipei, Taiwan

来源：

ANALYST | 2007年 / 132卷 / 01期

关键词：

D O I：

10.1039/b603611m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Bioaerosol detection in real time is an urgent civilian and military requirement. In this article, bioaerosol mass spectrometry, an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence, real-time qPCR, and FCM/FL were discussed. Although, challenging work remains to determine the interfering substances (e.g. particulates) of different environments, distinguish the specific species with specific probe, and overcome the high detection limit of FCM (10(4)-10(8) cells ml(-1)), literature reports suggested that FCM/FL has a great potential for real-time monitoring of bioaerosols.

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收藏

页码：14 / 16

页数：3

相关论文

共 18 条

[1] Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays [J].

Broccolo, F ;

Scarpellini, P ;

Locatelli, G ;

Zingale, A ;

Brambilla, AM ;

Cichero, P ;

Sechi, LA ;

Lazzarin, A ;

Lusso, P ;

Malnati, MS .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4565-4572

[2] Quantification of airborne Mycobacterium tuberculosis in health care setting using real-time qPCR coupled to an air-sampling filter method [J].

Chen, PS ;

Li, CS .

AEROSOL SCIENCE AND TECHNOLOGY, 2005, 39 (04) :371-376

[3] Sampling performance for bioaerosols by flow cytometry with fluorochrome [J].

Chen, PS ;

Li, CS .

AEROSOL SCIENCE AND TECHNOLOGY, 2005, 39 (03) :231-237

[4] Real-time quantitative PCR with gene probe, fluorochrome and flow cytometry for microorganism analysis [J].

Chen, PS ;

Li, CS .

JOURNAL OF ENVIRONMENTAL MONITORING, 2005, 7 (03) :257-262

[5] Bioaerosol characterization by flow cytometry with fluorochrome [J].

Chen, PS ;

Li, CS .

JOURNAL OF ENVIRONMENTAL MONITORING, 2005, 7 (10) :950-959

[6] Rapid and specific detection of Mycobacterium tuberculosis by using the smart cycler instrument and a specific fluorogenic probe [J].

Cleary, TJ ;

Roudel, G ;

Casillas, O ;

Miller, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4783-4786

[7] Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow Cytometry [J].

Day, JP ;

Kell, DB ;

Griffith, GW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (01) :37-45

[8] Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis [J].

Desjardin, LE ;

Chen, Y ;

Perkins, MD ;

Teixeira, L ;

Cave, MD ;

Eisenach, KD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1964-1968

[9] Reagentless detection and classification of individual bioaerosol particles in seconds [J].

Fergenson, DP ;

Pitesky, ME ;

Tobias, HJ ;

Steele, PT ;

Czerwieniec, GA ;

Russell, SC ;

Lebrilla, CB ;

Horn, JM ;

Coffee, KR ;

Srivastava, A ;

Pillai, SP ;

Shih, MTP ;

Hall, HL ;

Ramponi, AJ ;

Chang, JT ;

Langlois, RG ;

Estacio, PL ;

Hadley, RT ;

Frank, M ;

Gard, EE .

ANALYTICAL CHEMISTRY, 2004, 76 (02) :373-378

[10] Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence [J].

Hairston, PP ;

Ho, J ;

Quant, FR .

JOURNAL OF AEROSOL SCIENCE, 1997, 28 (03) :471-482