Analysis of Chromosomal Numbers, Mitochondrial Genome, and Full-Length Transcriptome of Onychostoma brevibarba

被引：8

作者：

Hu, Fangzhou ^{[1
,2
]}

Fan, Jingjing ^{[1
]}

Wu, Chang ^{[1
]}

Zhu, Ming ^{[1
]}

Zhou, Yunfan ^{[1
]}

Wang, Shi ^{[1
]}

Zhang, Chun ^{[1
]}

Tao, Min ^{[1
]}

Zhao, Rurong ^{[1
]}

Tang, Chenchen ^{[1
]}

Luo, Kaikun ^{[1
]}

Qin, Qinbo ^{[1
]}

Ma, Ming ^{[2
]}

Chen, Bo ^{[2
]}

Wang, Jinpu ^{[3
]}

Zhou, Aiguo ^{[4
]}

Bai, Liangxiong ^{[5
]}

Liu, Shaojun ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Hunan, Peoples R China

[2] Hunan Normal Univ, Key Lab Phytochem R&D Hunan Prov, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Hunan, Peoples R China

[3] Hunan Prov Livestock & Fisheries Bur, Changsha 410081, Hunan, Peoples R China

[4] Chenzhou Anim Husb Vet & Fisheries Bur, Changsha 410081, Hunan, Peoples R China

[5] Mangshan Forestry Resources Adm Bur, Changsha 410081, Hunan, Peoples R China

来源：

MARINE BIOTECHNOLOGY | 2019年 / 21卷 / 04期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Onychostoma brevibarba; Mitochondrial DNA; Chromosome and karyotype; Transcriptome; PacBio sequencing; EXPRESSION; TELEOSTEI; EVOLUTION; GENES; LINI;

D O I：

10.1007/s10126-019-09899-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Onychostoma brevibarba is a new discovered species which is distributed in Xiang Jiang River of the middle Chang Jiang basin in Hunan Province, South China. In this study, the ploidy levels of O. brevibarba were confirmed by counting chromosomal numbers and analyzing karyotype. The complete mitochondrial genome of O. brevibarba was determined and analyzed. Besides, we firstly performed the full-length transcriptome of O. brevibarba derived from 5 different tissues using the PacBio SMRT sequencing. The result shows that O. brevibarba was a diploid with 48 chromosomes. The complete mitogenome of O. brevibarba was 16,602 bp in size and very similar (89.1-91.3%) to that of other Onychostoma species but was distinct from all congeners. The full-length transcriptome dataset of O. brevibarba comprised 120,239 unigenes. Among the unigenes, 91,542 were functionally annotated, whereas 26,794 were found to have two or more isoforms. This study could provide many new insights into cytology and molecular characteristics of O. brevibarba; it laid the foundation for further exploration of the genomic signatures of species of Onychostoma.

引用

页码：515 / 525

页数：11

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