Application of T-RFLP analysis to the study of the coastal Black Sea bacterioplankton

被引:0
作者
Stoica, Elena [1 ]
机构
[1] Natl Inst Marine Res & Dev Grigore Antipa, Dept Ecol & Environm Protect, Constanta, Romania
来源
ROMANIAN BIOTECHNOLOGICAL LETTERS | 2009年 / 14卷 / 05期
关键词
bacterioplankton; Bacteria; Archaea; diversity; genetic fingerprinting method; T-RFLP; Black Sea; 16S RIBOSOMAL-RNA; LENGTH-POLYMORPHISM ANALYSIS; COMMUNITY STRUCTURE; BACTERIAL COMMUNITIES; MARINE BACTERIOPLANKTON; WATER-COLUMN; RESTRICTION; DIVERSITY; GENES; PROFILES;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Marine bacterioplankton play important roles in biogeochemical cycles in coastal regions of the sea. Despite its ecological importance, the composition of bacterioplankton community of coastal Black Sea, a unique marine basin in the World, is poorly characterized Thus. the aim of this study was to analyze the bacterial communities in the Black Sea surface waters by terminal-restriction fragment length polymorphism (T-RFLP) analysis, a relatively new, fast and easy molecular method for assessing microbial diversity and community structure. Bacterioplankton assemblages were sampled in shelf surface waters in Constanta Bay between May and August 2004. T-RFLP patterns derived from enzyme digestion with HhaI were analyzed in order to provide a preliminary, picture of the relative diversity of this complex microbial community. Members of both prokaryote domains, Archaea and Bacteria, have been successfully identified using T-RFLP analysis of amplified total community 16S rDNA from surface seawaters and were related to the environmental conditions. A minimum of 20 operational taxonomic units (OTU) were identified with T-RFLP. This study contributes to the evaluation of the Black Sea bacterioplankton diversity and it was used for the first lime in Romania.
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页码:4710 / 4719
页数:10
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