Citric acid functionalized silane coupling versus post-grafting strategy for dual pH and saline responsive delivery of cisplatin by Fe3O4/carboxyl functionalized mesoporous SiO2 hybrid nanoparticles: A-synthesis, physicochemical and biological characterization

被引：33

作者：

Abedi, Mehdi ^{[1
]}

Abolmaali, Samira Sadat ^{[1
,2
]}

Abedanzadeh, Mozhgan ^{[1
]}

Borandeh, Sedigheh ^{[2
]}

Samani, Soliman Mohammadi ^{[2
,3
]}

Tamaddon, Ali Mohammad ^{[1
,2
]}

机构：

[1] Shiraz Univ Med Sci, Sch Pharm, Dept Pharmaceut Nanotechnol, Shiraz, Iran

[2] Shiraz Univ Med Sci, Sch Pharm, Ctr Nanotechnol Drug Delivery, Shiraz, Iran

[3] Shiraz Univ Med Sci, Sch Pharm, Dept Pharmaceut, Shiraz, Iran

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2019年 / 104卷

关键词：

Cisplatin; Breast cancer; Core-shell; Magnetic nanoparticle; Mesoporous silica; Citric acid; SILICA NANOPARTICLES; IN-VITRO; SURFACE FUNCTIONALITY; POLY ETHYLENEIMINE; ANTITUMOR-ACTIVITY; CONTROLLED-RELEASE; CO-CONDENSATION; CELLULAR UPTAKE; GRAPHENE OXIDE; PROTEIN CORONA;

D O I：

10.1016/j.msec.2019.109922

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Synthesis of monodisperse carboxylic acid-functionalized magnetic mesoporous silica nanoparticles is performed by either two-step sol-gel process or post-grafting using citric acid modified isocyanate silane coupling agent (MMSN-NCO-CA) or succinic anhydride modified magnetic mesoporous silica (MMSN-NH-SA). Morphology, structure and magnetic properties of bare and mesoporous silica coated Fe3O4 core were studied using various techniques such as FTIR, VSM, TEM, FESEM, XRD and NZ adsorption-desorption isotherms (BET). Cisplatin (cis-Pt) adsorption isotherms and its release profile in various media were investigated by ICP-OES. MMSN-NCO-CA with mean particle size 107 nm had lower surface area (87.5 m(2)/g) and larger pore size (6.9 rim) in comparison with MMSN-NH-SA (respective values of 151.2 m(2)/g and 3.5 nm). cis-Pt loading into particles followed a saturable adsorption with respect to the drug to particle mass ratios. More sustained release of cis-Pt was observed for MMSN-NCO-CA, though both nanoparticles exhibited a pH- and saline concentration-dependent drug release. In addition, general and cis-Pt specific cytotoxicity were examined by MTT assay in MDA-MB-231 breast cancer cell line, and to further detect apoptosis, acridine orange/ethidium bromide dual cell staining was investigated by fluorescence microscopy. In-vitro anti-tumor efficiency of cis-Pt loaded MMSN-NCO-CA and MMSN-NH-SA were similarly enhanced in comparison to free cis-Pt; however, more specific apoptotic death occurred for cis-Pt loaded MMSN-NCO-CA. Therefore, the as-synthesized citric acid functionalized core-shell magnetic mesoporous hybrid nanoparticles could be used as a promising drug carriers for cancer therapy in-vivo.

引用

页数：12