Isolation and characterization of normal hamster buccal pouch stem/stromal cells - A potential oral cancer stem/stem-like cell model

被引：8

作者：

Huang, Anderson Hsien-Cheng ^{[1
,2
]}

Chen, Yuk-Kwan ^{[1
,3
,4
]}

Chan, Anthony Wing-Sang ^{[5
]}

Shieh, Tien-Yu ^{[6
,7
]}

Lin, Li-Min ^{[1
,3
]}

机构：

[1] Kaohsiung Med Univ, Coll Dent Med, Dept Oral Pathol, Fac Dent, Kaohsiung 807, Taiwan

[2] Kaohsiung Med Univ, Coll Dent Med, Sch Dent, Kaohsiung 807, Taiwan

[3] Kaohsiung Med Univ Hosp, Dept Oral Pathol, Div Dent, Kaohsiung, Taiwan

[4] Kaohsiung Med Univ, Ctr Excellence Environm Med, Kaohsiung 807, Taiwan

[5] Emory Univ, Sch Med, Dept Human Genet, Yerkes Natl Primate Res Ctr, Atlanta, GA USA

[6] Kaohsiung Med Univ, Coll Dent Med, Fac Dent, Dept Oral & Maxillofacial Surg, Kaohsiung 807, Taiwan

[7] Kaohsiung Med Univ Hosp, Dept Oral & Maxillofacial Surg, Div Dent, Kaohsiung, Taiwan

来源：

ORAL ONCOLOGY | 2009年 / 45卷 / 11期

关键词：

Cancer stem cell; Stem cell; Hamster buccal pouch; Oral cancer; GENETIC PROGRESSION MODEL; STEM-LIKE CELLS; DENTAL-PULP; NECK-CANCER; CARCINOMA; HEAD; IDENTIFICATION; TAIWAN; MUCOSA;

D O I：

10.1016/j.oraloncology.2009.07.003

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The hamster buccal pouch (HBP) is an appropriate experimental model for buccal squamous cell carcinoma (SCC). Our objective was to isolate and characterize the stem/stromal cells from normal HBP. HBP stem/stromal cells were successfully derived from three of five normal pouch tissues, which differentiated into adipogenic, chondrogenic, and osteogenic lineages, and also expressed stem cell and differentiation markers, indicating their stem cell origin and differentiation capability. These cells showed high expression of CD29, CD90, and CD105, markers specific for bone marrow stem cells, and exhibited very low expression of CD14, CD34, and CD45, markers specific for hematopoietic cells. Of the HBP stem/stromal cells isolated, 90% stained positively for cytoplasmic keratin, whereas 10% stained positively for vimentin. In conclusion, normal HBP stem/stromal cells provide a potential avenue for future experimental trials of cancer stem/stem-like cells for treatment of buccal SCC. In vitro, we may detect the sequential changes of normal HBP stem/stromal cells during multistep oral carcinogenesis or the alternations of these cells upon irradiation treatment and/or chemotherapy. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：E189 / E195

页数：7

共 29 条

[1] Prospective identification of tumorigenic breast cancer cells
Al-Hajj, M
Wicha, MS
Benito-Hernandez, A
Morrison, SJ
Clarke, MF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) : 3983 - 3988
[2] Braakhuis BJM, 2004, J ORAL PATHOL MED, V33, P317
[3] Califano J, 1996, CANCER RES, V56, P2488
[4] Primary oral squamous cell carcinoma: an analysis of 703 cases in southern Taiwan
Chen, YK
Huang, HC
Lin, LM
Lin, CC
[J]. ORAL ONCOLOGY, 1999, 35 (02) : 173 - 179
[5] Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee
Cheng, Pei-Hsun
Snyder, Brooke
Fillos, Dimitri
Ibegbu, Chris C.
Huang, Anderson Hsien-Cheng
Chan, Anthony W. S.
[J]. BMC CELL BIOLOGY, 2008, 9 (1)
[6] Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
Chiou, Shih-Hwa
Yu, Cheng-Chia
Huang, Chi-Yang
Lin, Shu-Chun
Liu, Chung-Ji
Tsai, Tung-Hu
Chou, Shiu-Huey
Chien, Chian-Shiu
Ku, Hung-Hai
Lo, Jeng-Fan
[J]. CLINICAL CANCER RESEARCH, 2008, 14 (13) : 4085 - 4095
[7] Carcinoma of the buccal mucosa: Analysis of clinical presentation, outcome and prognostic factors
Ghoshal, Sushmita
Mallick, Indranil
Panda, Naresh
Sharma, Suresh C.
[J]. ORAL ONCOLOGY, 2006, 42 (05) : 533 - 539
[8] GIMENEZCONTI IB, 1993, J CELL BIOCHEM, P83
[9] The stem cell hypothesis in head and neck cancer
Graziano, A.
d'Aquino, R.
Tirino, V.
Desiderio, V.
Rossi, A.
Pirozzi, G.
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2008, 103 (02) : 408 - 412
[10] Gros J, 1999, J CELL SCI, V112, P3791

← 1 2 3 →