MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly

被引：199

作者：

Franz, Cerstin ^{[1
]}

Walczak, Rudolf ^{[1
]}

Yavuz, Sevil ^{[1
]}

Santarella, Rachel ^{[1
]}

Gentzel, Marc ^{[1
]}

Askjaer, Peter ^{[1
]}

Galy, Vincent ^{[1
]}

Hetzer, Martin ^{[1
]}

Mattaj, Iain W. ^{[1
]}

Antonin, Wolfram ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

EMBO REPORTS | 2007年 / 8卷 / 02期

关键词：

mitosis; kinetochore; nuclear envelope assembly; nuclear pore complex; Nup107-160; complex; MEL-28;

D O I：

10.1038/sj.embor.7400889

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metazoan nuclear envelope (NE) breaks down and re-forms during each cell cycle. Nuclear pore complexes (NPCs), which allow nucleocytoplasmic transport during interphase, assemble into the re-forming NE at the end of mitosis. Using in vitro NE assembly, we show that the vertebrate homologue of MEL-28 (maternal effect lethal), a recently discovered NE component in Caenorhabditis elegans, functions in postmitotic NPC assembly. MEL-28 interacts with the Nup107-160 complex (Nup for nucleoporin), an important building block of the NPC, and is essential for the recruitment of the Nup107-160 complex to chromatin. We suggest that MEL-28 acts as a seeding point for NPC assembly.

引用

页码：165 / 172

页数：8

共 48 条

[31] Determining the order of nucleoporin recruitment during Nuclear Pore Complex Assembly.
Thaller, D.
Colombi, P.
Lusk, P.
MOLECULAR BIOLOGY OF THE CELL, 2016, 27
[32] Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly
Zhang, CM
Goldberg, MW
Moore, WJ
Allen, TD
Clarke, PR
EUROPEAN JOURNAL OF CELL BIOLOGY, 2002, 81 (11) : 623 - 633
[33] The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly
Makio, Tadashi
Stanton, Leslie H.
Lin, Cheng-Chao
Goldfarb, David S.
Weis, Karsten
Wozniak, Richard W.
JOURNAL OF CELL BIOLOGY, 2009, 185 (03): : 459 - 473
[34] Dunking into the Lipid Bilayer: How Direct Membrane Binding of Nucleoporins Can Contribute to Nuclear Pore Complex Structure and Assembly
Hamed, Mohamed
Antonin, Wolfram
CELLS, 2021, 10 (12)
[35] Members of the RSC Chromatin-Remodeling Complex Are Required for Maintaining Proper Nuclear Envelope Structure and Pore Complex Localization
Titus, Laura C.
Dawson, T. Renee
Rexer, Deborah J.
Ryan, Kathryn J.
Wente, Susan R.
MOLECULAR BIOLOGY OF THE CELL, 2010, 21 (06) : 1072 - 1087
[36] The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function
Madrid, AS
Mancuso, J
Cande, WZ
Weis, K
JOURNAL OF CELL BIOLOGY, 2006, 173 (03): : 361 - 371
[37] Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly
Funakoshi, Tomoko
Clever, Michaela
Watanabe, Ai
Imamoto, Naoko
MOLECULAR BIOLOGY OF THE CELL, 2011, 22 (07) : 1058 - 1069
[38] Yeast Nle3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex
Kenna, MA
Petranka, JG
Reilly, JL
Davis, LI
MOLECULAR AND CELLULAR BIOLOGY, 1996, 16 (05) : 2025 - 2036
[39] Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly, and chromatin de-condensation
Allana Schooley
Benjamin Vollmer
Wolfram Antonin
Chromosoma, 2012, 121 : 539 - 554
[40] The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells
Mansfeld, J
Güttinger, S
Hawryluk-Gara, LA
Panté, N
Mall, M
Galy, V
Haselmann, U
Mühlhäusser, P
Wozniak, RW
Mattaj, IW
Kutay, U
Antonin, W
MOLECULAR CELL, 2006, 22 (01) : 93 - 103

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