Isolation and characterization of fifteen polymorphic microsatellite loci from Primula merrilliana (Primulaceae), an endemic from China

被引：9

作者：

Peng, Yan-qiu ^{[1
,2
]}

Shao, Jian-wen ^{[1
,2
]}

Wu, Hai-long ^{[1
,2
]}

Zhang, Xiao-ping ^{[1
,2
]}

Zhu, Guo-ping ^{[1
,2
]}

机构：

[1] Anhui Normal Univ, Coll Life Sci, Key Lab Mol Evolut & Biodivers, Wuhu 241000, Peoples R China

[2] Anhui Normal Univ, Key Lab Biot Environm & Ecol Safety Anhui Prov, Wuhu 241000, Peoples R China

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Primula merrilliana; Microsatellites; Conservation; Population genetics; GENETIC DIVERSITY; POPULATION-SIZE;

D O I：

10.1007/s10592-008-9756-1

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Primula merrilliana (Primulaceae) is an endangered plant endemic to south Anhui Province in China. In this study, we isolated fifteen polymorphic microsatellite loci from a population of 22 individuals using a modified biotin-capture method. Our isolated loci provided microsatellite markers with polymorphism of 311 alleles per locus. The expected and observed heterozygosities ranged from 0.392 to 0.855 and from 0.136 to 1.000, respectively. These markers would be the useful tools for analyzing questions concerning population genetic structure and mating system of Primula merrilliana.

引用

页码：1441 / 1443

页数：3

共 15 条

[1] Microsatellite analysis of demographic genetic structure in fragmented populations of the tropical tree Symphonia globulifera [J].