Peptide translocators with engineered dehydration-prone hydrogen bonds

被引：8

作者：

Maddipati, Sridhar

Fernandez, Ariel ^{[1
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

[2] Univ Texas, MD Anderson Canc Ctr, Dept Expt Therapeut, Houston, TX 77030 USA

[3] Purdue Univ, Sch Chem Engn, W Lafayette, IN 47906 USA

来源：

JOURNAL OF CHEMICAL PHYSICS | 2007年 / 126卷 / 06期

关键词：

D O I：

10.1063/1.2698277

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The unfavorable crossing of cell membranes by water-soluble peptides poses a challenge to peptide-based drug delivery. Thus the authors engineered a conformational switch that modulates cellular translocation. The peptide design hinges on dehydration propensities of hydrogen bonds that form to mitigate polar-group internalization penalties and is experimentally corroborated through cell-internalization assays. (c) 2007 American Institute of Physics.

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页数：5

相关论文

共 19 条

[1] In search of the energetic role of peptide hydrogen bonds [J].

Baldwin, RL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (20) :17581-17588

[2] CONFORMATION OF GLUCAGON IN A LIPID WATER INTERPHASE BY H-1 NUCLEAR MAGNETIC-RESONANCE [J].

BRAUN, W ;

WIDER, G ;

LEE, KH ;

WUTHRICH, K .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 169 (04) :921-948

[3] Keeping dry and crossing membranes [J].

Fernández, A .

NATURE BIOTECHNOLOGY, 2004, 22 (09) :1081-1084

[4] Adherence of packing defects in soluble proteins -: art. no. 018102 [J].

Fernández, A ;

Scott, LR .

PHYSICAL REVIEW LETTERS, 2003, 91 (01)

[5] Proteins with H-bond packing defects are highly interactive with lipid bilayers:: Implications for amyloidogenesis [J].

Fernández, A ;

Berry, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2391-2396

[6] Dynamics of hydrogen bond desolvation in protein folding [J].

Fernández, A ;

Sosnick, TR ;

Colubri, A .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 321 (04) :659-675

[7] Insufficiently dehydrated hydrogen bonds as determinants of protein interactions [J].

Fernández, A ;

Scheraga, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (01) :113-118

[8] Arginine-rich peptides - An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery [J].

Futaki, S ;

Suzuki, T ;

Ohashi, W ;

Yagami, T ;

Tanaka, S ;

Ueda, K ;

Sugiura, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (08) :5836-5840

[9]

HENDSCH ZS, 1994, PROTEIN SCI, V3, P211

[10] FREE-ENERGY CALCULATIONS - APPLICATIONS TO CHEMICAL AND BIOCHEMICAL PHENOMENA [J].

KOLLMAN, P .

CHEMICAL REVIEWS, 1993, 93 (07) :2395-2417