The Application of a Bone Marrow Mesenchymal Stem Cell Membrane in the Vascularization of a Decellularized Tracheal Scaffold

被引:8
作者
Yao, Zhi-Ye [1 ]
Feng, Bo-Wen [1 ,2 ]
Liu, Cai-Sheng [1 ,3 ]
Liu, Yu-Mei [1 ]
Zhou, Hai-Yu [4 ]
Zhang, Xiao-Hui [1 ,5 ]
Jian, Min-Qiao [1 ,3 ]
Mo, Jian-Ling [1 ,2 ]
Liang, Yi-Jing [1 ,5 ]
Chen, Liang [1 ]
Liu, Xiao-Qing [6 ]
Chen, Yan-Ling [1 ]
Zhang, Zhan-Song [1 ]
He, Shao-Ru [1 ,4 ]
机构
[1] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Dept Neonatol, Guangzhou 510030, Peoples R China
[2] Southern Med Univ, Sch Clin Med 2, Guangzhou 510515, Peoples R China
[3] South China Univ Technol, Med Coll, Guangzhou 510641, Peoples R China
[4] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Dept Thorac Surg, Guangzhou 510030, Peoples R China
[5] Guangdong Prov Cardiovasc Inst, Dept Cardiol, Guangzhou 510030, Peoples R China
[6] Guangdong Prov Peoples Hosp, Dept Epidemiol, Guangzhou 510030, Peoples R China
关键词
ASCORBIC-ACID; EPITHELIAL-CELLS; ENDOTHELIAL-CELLS; TISSUE; SHEET; MATRIX; ANGIOGENESIS; EXPRESSION; CULTURE; DIFFERENTIATION;
D O I
10.1155/2021/6624265
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Airway stenosis is a common problem in the neonatal intensive care unit (NICU) and pediatric intensive care unit (PICU). A tissue-engineered trachea is a new therapeutic method and a research hotspot. Successful vascularization is the key to the application of a tissue-engineered trachea. However, successful vascularization studies lack a complete description. In this study, it was assumed that rabbit bone marrow mesenchymal stem cells were obtained and induced by ascorbic acid to detect the tissue structure, ultrastructure, and gene expression of the extracellular matrix. A vascular endothelial cell culture medium was added in vitro to induce the vascularization of the stem cell sheet (SCS), and the immunohistochemistry and gene expression of vascular endothelial cell markers were detected. At the same time, vascular growth-related factors were added and detected during SCS construction. After the SCS and decellularized tracheal (DT) were constructed, a tetrandrine allograft was performed to observe its vascularization potential. We established the architecture and identified rabbit bone marrow mesenchymal stem cell membranes by 14 days of ascorbic acid, studied the role of a vascularized membrane in inducing bone marrow mesenchymal stem cells by in vitro ascorbic acid, and assessed the role of combining the stem cell membranes and noncellular tracheal scaffolds in vivo. Fourteen experiments confirmed that cell membranes promote angiogenesis at gene level. The results of 21-day in vitro experiments showed that the composite tissue-engineered trachea had strong angiogenesis. In vivo experiments show that a composite tissue-engineered trachea has strong potential for angiogenesis. It promotes the understanding of diseases of airway stenosis and tissue-engineered tracheal regeneration in newborns and small infants.
引用
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页数:16
相关论文
共 77 条
[1]   Current Solutions for Long-Segment Tracheal Reconstruction [J].
Abouarab, Ahmed A. ;
Elsayed, Hany H. ;
Elkhayat, Hussein ;
Mostafa, Ahmed ;
Cleveland, David C. ;
El Nori, Ahmed .
ANNALS OF THORACIC AND CARDIOVASCULAR SURGERY, 2017, 23 (02) :66-75
[2]   Ultrathin poly(N-isopropylacrylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control [J].
Akiyama, Y ;
Kikuchi, A ;
Yamato, M ;
Okano, T .
LANGMUIR, 2004, 20 (13) :5506-5511
[3]   Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays [J].
Amano, Yuto ;
Nishiguchi, Akihiro ;
Matsusaki, Michiya ;
Iseoka, Hiroko ;
Miyagawa, Shigeru ;
Sawa, Yoshiki ;
Seo, Manabu ;
Yamaguchi, Takashi ;
Akashi, Mitsuru .
ACTA BIOMATERIALIA, 2016, 33 :110-121
[4]  
[Anonymous], 2019, CLIN PHARMACOL THER, V106
[5]   Ascorbic acid: much more than just an antioxidant [J].
Arrigoni, O ;
De Tullio, MC .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1569 (1-3) :1-9
[6]   Outcomes of slide tracheoplasty in 101 children: A 17-year single-center experience [J].
Butler, Colin R. ;
Speggiorin, Simone ;
Rijnberg, Friso M. ;
Roebuck, Derek J. ;
Muthialu, Nagarajan ;
Hewitt, Richard J. ;
Elliott, Martin J. .
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2014, 147 (06) :1783-1789
[7]   Cell sheet technology-driven re-epithelialization and neovascularization of skin wounds [J].
Cerqueira, M. T. ;
Pirraco, R. P. ;
Martins, A. R. ;
Santos, T. C. ;
Reis, R. L. ;
Marques, A. P. .
ACTA BIOMATERIALIA, 2014, 10 (07) :3145-3155
[8]   A Novel Method to Acquire Decellularized Tracheal Matrix from Neonatal Rabbit [J].
Chen, Liang ;
He, Shao-Ru ;
Zhuang, Jian ;
Sun, Yun-Xia ;
Liang, Sui-Xin ;
Liu, Yu-Mei ;
Wang, Yi-Fei ;
Sun, Xin ;
Chen, Yan-Ling .
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2016, 6 (05) :400-407
[9]   Bone Marrow Mesenchymal Stem Cells Differentiate Into Functional Tracheal Epithelial Cells by Air-Liquid Interface Culture Conditions [J].
Chen, Yan-Ling ;
He, Shao-Ru ;
Zhuang, Jian ;
Chen, Liang ;
Wang, Yi-Fei ;
Liu, Yu-Mei ;
Yao, Zhi-Ye .
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2016, 6 (08) :613-620
[10]  
Cheng Vinton, 2015, Oncoscience, V2, P596