The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases

被引：27

作者：

Kenyon, Johanna J. ^{[1
]}

Arbatsky, Nikolay P. ^{[2
]}

Shneider, Mikhail M. ^{[3
]}

Popova, Anastasiya V. ^{[4
,5
]}

Dmitrenok, Andrei S. ^{[2
]}

Kasimova, Anastasiya A. ^{[2
,6
]}

Shashkov, Alexander S. ^{[2
]}

Hall, Ruth M. ^{[7
]}

Knirel, Yuriy A. ^{[2
]}

机构：

[1] Queensland Univ Technol, Fac Hlth, Sch Biomed Sci, Inst Hlth & Biomed Innovat, Brisbane, Qld, Australia

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow, Russia

[3] Russian Acad Sci, MM Shemyakin & YA Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[4] Moscow Inst Phys & Technol, Dolgoprudnyi, Moscow Region, Russia

[5] State Res Ctr Appl Microbiol & Biotechnol, Obolensk, Moscow Region, Russia

[6] DI Mendeleev Univ Chem Technol Russia, Russian Acad Sci, Higher Chem Coll, Moscow, Russia

[7] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia

来源：

PLOS ONE | 2019年 / 14卷 / 06期

基金：

俄罗斯科学基金会; 澳大利亚研究理事会;

关键词：

GENE-CLUSTER; ANTIGEN; UNIT; GLUCOSYLATION; BIOSYNTHESIS;

D O I：

10.1371/journal.pone.0218461

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Acinetobacter baumannii isolate NIPH 329 carries a novel capsular polysaccharide (CPS) gene cluster, designated KL46, that is closely related to the KL5 locus in A. baumannii isolate SDF but includes genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid (Pse5Ac7Ac) instead of the corresponding D-glycero-D-galacto isomer (di-N-acetyllegionaminic acid) (Leg5Ac7Ac). In agreement with the genetic content of KL46, chemical studies of the K46 CPS produced by NIPH 329 revealed a branched tetrasaccharide repeat (K unit) with an overall structure the same as K5 from SDF but with (a) over cap -Pse5Ac7Ac replacing alpha-Leg5Ac7Ac. As for K5, the K46 unit begins with D-GalpNAc and includes alpha-D-GlcpNAc-(1 -> 3)-D-GalpNAc and alpha-D-Galp-(1 -> 6)-D-GlcpNAc linkages, formed by Gtr14 and Gtr15 glycosyltransferases, respectively. The Gtr94(K46) glycosyltransferase, which is related to Gtr13(K5), links Pse5Ac7Ac to D-Galp in the growing K unit via a (a) over cap-(2 -> 6) linkage. Nearly identical Wzy enzymes connect the K46 and K5 units via a alpha-D-GalpNAc-(1 -> 3)-alpha-D-Galp linkage to form closely related CPSs. Both Pse5Ac7Ac in K46 and Leg5Ac7Ac in K5 are acetylated at O4 but no acetyltransferase gene is present in KL46 or KL5. Related acetyltransferases were found encoded in the NIPH 329 and SDF genomes, but not in other strains carrying an unacetylated Pse or Leg derivative in the CPS. The genes encoding the acetyltransferases were in different putative phage genomes. However, related acetyltransferases were rare among the > 3000 publically available genome sequences.

引用

页数：13

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