Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques

被引：48
|
作者：
Martínez-Ramírez, A [1 ]
Rodríguez-Perales, S [1 ]
Meléndez, B [1 ]
Martínez-Delgado, B [1 ]
Urioste, M [1 ]
Cigudosa, JC [1 ]
Benítez, J [1 ]
机构：
[1] Inst Salud Carlos III, Spanish Natl Canc Ctr, CNIO,Mol Pathol Program, Dept Human Genet, Madrid, Spain
来源：
CANCER GENETICS AND CYTOGENETICS | 2003年 / 141卷 / 02期
关键词：
D O I：
10.1016/S0165-4608(02)00670-2
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The A673 cell line was established from a patient with a primary rhabdomyosarcoma (RMS), which is referred to in the literature either as a Ewing tumor (ET) or as RMS. Although the two tumoral types are associated with specific and well-characterized translocations, no cytogenetic report on this cell line has been published. We characterized the A673 cell line using a combination of spectral karyotyping (SKY), fluorescence in situ hybridization (FISH), and reverse transcriptase polymerase chain reaction (RT-PCR), which revealed the presence of a complex karyotype and a translocation involving chromosomes 11 and 22 and the fusion of EWS and FL11 genes, both events being specific to ET. Neither cytogenetics nor molecular alterations specific to RMS were found. (C) 2003 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：138 / 142
页数：5
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