Multiple quantitative trait loci contribute to resistance to bacterial canker incited by Pseudomonas syringae pv. actinidiae in kiwifruit (Actinidia chinensis)

被引：36

作者：

Tahir, Jibran ^{[1
]}

Hoyte, Stephen ^{[2
]}

Bassett, Heather ^{[1
]}

Brendolise, Cyril ^{[3
]}

Chatterjee, Abhishek ^{[3
]}

Templeton, Kerry ^{[3
]}

Deng, Cecilia ^{[3
]}

Crowhurst, Ross ^{[3
]}

Montefiori, Mirco ^{[4
]}

Morgan, Ed ^{[1
]}

Wotton, Andrew ^{[1
]}

Funnell, Keith ^{[1
]}

Wiedow, Claudia ^{[1
]}

Knaebel, Mareike ^{[1
]}

Hedderley, Duncan ^{[1
]}

Vanneste, Joel ^{[2
]}

McCallum, John ^{[5
]}

Hoeata, Kirsten ^{[6
]}

Nath, Amardeep ^{[6
]}

Chagne, David ^{[1
]}

Gea, Luis ^{[6
]}

Gardiner, Susan E. ^{[1
]}

机构：

[1] New Zealand Inst Plant & Food Res Ltd, Manawatu Mai & Ctr, Private Bag 11030, Palmerston North 4442, New Zealand

[2] New Zealand Inst Plant Food Res Ltd, Hamilton, New Zealand

[3] New Zealand Inst Plant & Food Res Ltd, Private Bag 92-169, Auckland 1025, New Zealand

[4] New Plant, Via Malpighi 5, I-47122 Forli, Italy

[5] New Zealand Inst Plant & Food Res Ltd, Lincoln, New Zealand

[6] New Zealand Inst Plant & Food Res Ltd, 412 1 Rd,RD2, Te Puke 3182, New Zealand

来源：

HORTICULTURE RESEARCH | 2019年 / 6卷

关键词：

NEW-ZEALAND; ARABIDOPSIS; DEFENSE; PATHOGEN; SURVIVAL; DISEASE; PSA; DURABILITY; EVOLUTION; OUTBREAK;

D O I：

10.1038/s41438-019-0184-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pseudomonas syringae pv. actinidiae (Psa) biovar 3, a virulent, canker-inducing pathogen is an economic threat to the kiwifruit (Actinidia spp.) industry worldwide. The commercially grown diploid (2x) A. chinensis var. chinensis is more susceptible to Psa than tetraploid and hexaploid kiwifruit. However information on the genetic loci modulating Psa resistance in kiwifruit is not available. Here we report mapping of quantitative trait loci (QTLs) regulating resistance to Psa in a diploid kiwifruit population, derived from a cross between an elite Psa-susceptible 'Hort16A' and a resistant male breeding parent P1. Using high-density genetic maps and intensive phenotyping, we identified a single QTL for Psa resistance on Linkage Group (LG) 27 of 'Hort16A' revealing 16-19% phenotypic variance and candidate alleles for susceptibility and resistance at this loci. In addition, six minor QTLs were identified in P1 on distinct LGs, exerting 4-9% variance. Resistance in the Fl population is improved by additive effects from 'Hort16A' and P1 QTLs providing evidence that divergent genetic pathways interact to combat the virulent Psa strain. Two different bioassays further identified new QTLs for tissue-specific responses to Psa. The genetic marker at LG27 QTL was further verified for association with Psa resistance in diploid Actinidia chinensis populations. Transcriptome analysis of Psa-resistant and susceptible genotypes in field revealed hallmarks of basal defense and provided candidate RNA-biomarkers for screening for Psa resistance in greenhouse conditions.

引用

页数：18

共 94 条

[1] Current situation and characterization of Pseudomonas syringae pv. actinidiae on kiwifruit in Galicia (northwest Spain) [J].

Abelleira, A. ;

Ares, A. ;

Aguin, O. ;

Picoaga, A. ;

Lopez, M. M. ;

Mansilla, P. .

PLANT PATHOLOGY, 2014, 63 (03) :691-699

[2]

[Anonymous], 1994, KOREAN J PLANT PATHO

[3] Current status of bacterial canker spread on kiwifruit in Italy [J].

Balestra G.M. ;

Mazzaglia A. ;

Quattrucci A. ;

Renzi M. ;

Rossetti A. .

Australasian Plant Disease Notes, 2009, 4 (1) :34-36

[4] Evolution, genomics and epidemiology of Pseudomonas syringae: Challenges in Bacterial Molecular Plant Pathology [J].

Baltrus, David A. ;

McCann, Honour C. ;

Guttman, David S. .

MOLECULAR PLANT PATHOLOGY, 2017, 18 (01) :152-168

[5] Mandipropamid targets the cellulose synthase-like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans [J].

Blum, Mathias ;

Boehler, Martine ;

Randall, Eva ;

Young, Vanessa ;

Csukai, Michael ;

Kraus, Sabrina ;

Moulin, Florence ;

Scalliet, Gabriel ;

Avrova, Anna O. ;

Whisson, Stephen C. ;

Fonne-Pfister, Raymonde .

MOLECULAR PLANT PATHOLOGY, 2010, 11 (02) :227-243

[6] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

[7] R/qtl: QTL mapping in experimental crosses [J].

Broman, KW ;

Wu, H ;

Sen, S ;

Churchill, GA .

BIOINFORMATICS, 2003, 19 (07) :889-890

[8] Durable Resistance of Crops to Disease: A Darwinian Perspective [J].

Brown, James K. M. .

ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 53, 2015, 53 :513-539

[9] Quantitative resistance increases the durability of qualitative resistance to Leptosphaeria maculans in Brassica napus [J].

Brun, Hortense ;

Chevre, Anne-Marie ;

Fitt, Bruce D. L. ;

Powers, Stephen ;

Besnard, Anne-Laure ;

Ermel, Magali ;

Huteau, Virginie ;

Marquer, Bruno ;

Eber, Frederique ;

Renard, Michel ;

Andrivon, Didier .

NEW PHYTOLOGIST, 2010, 185 (01) :285-299

[10]

Bus Vincent G. M., 2009, V6, P563, DOI 10.1007/978-0-387-77491-6_27

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