Reverse micellar extraction for downstream processing of lipase: Effect of various parameters on extraction

被引：53

作者：

Nandini, K. E. ^{[1
]}

Rastogi, Navin K. ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Dept Food Engn, Mysore 570020, Karnataka, India

来源：

PROCESS BIOCHEMISTRY | 2009年 / 44卷 / 10期

关键词：

Reverse micellar extraction; Downstream processing; Lipase; Cationic surfactant; Activity recovery; Extraction efficiency; ALPHA-AMYLASE; ASPERGILLUS-NIGER; BACK-EXTRACTION; PURIFICATION; SYSTEM; AOT; PROTEINS/ENZYMES; MICROEMULSIONS; FERMENTATION; SURFACTANT;

D O I：

10.1016/j.procbio.2009.06.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reverse micellar extraction of lipase using cationic surfactant cetyltrimethylammonium bromide (CTAB) was investigated. The effect of various process parameters on both forward and backward extraction of lipase from crude extract was studied to optimize its yield and purity. Forward extraction of lipase was found to be maximum using Tris buffer at pH 9.0 containing 0.10 M NaCl in aqueous phase and 0.20 M CTAB in organic phase consisting of isooctane, butanol and hexanol. In case of backward extraction, lipase was extracted from the organic phase to a fresh aqueous phase in 0.05 M potassium phosphate buffer (pH 7,0) containing 1.0 M KCl. The activity recovery, extraction efficiency and purification factor of lipase were found to be 82.72%, 40.27% and 4.09-fold, respectively. The studies also indicated that the organic phase recovered after back extraction could be reused for the extraction of lipase from crude extract. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1172 / 1178

页数：7

共 43 条

[31] Higher order structure of Mucor miehei lipase and micelle size in cetyltrimethylammonium bromide reverse micellar system [J].

Naoe, Kazumitsu ;

Takeuchi, Chihiro ;

Kawagoe, Mikio ;

Nagayama, Kazuhito ;

Imai, Masanao .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 850 (1-2) :277-284

[32] One-step separation of lysozyme by reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide [J].

Noh, KH ;

Imm, JY .

FOOD CHEMISTRY, 2005, 93 (01) :95-101

[33] Recovery of inulinase using BDBAC reversed micelles [J].

Pessoa, A ;

Vitolo, M .

PROCESS BIOCHEMISTRY, 1998, 33 (03) :291-297

[34] Studies on the purification of peroxidase from horseradish roots using reverse micelles [J].

Regalado, C ;

Asenjo, JA ;

Pyle, DL .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (05) :332-339

[35] Production, purification, characterization, and applications of lipases [J].

Sharma, R ;

Chisti, Y ;

Banerjee, UC .

BIOTECHNOLOGY ADVANCES, 2001, 19 (08) :627-662

[36]

[沈睿 Shen Rui], 2005, [过程工程学报, The chinese journal of process engineering], V5, P255

[37] Selective precipitation and recovery of xylanase using surfactant and organic solvent [J].

Shin, YO ;

Wahnon, D ;

Weber, ME ;

Vera, JH .

BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (06) :698-705

[38] Purification and partial characterization of a lipase from Antrodia cinnamomea [J].

Shu, CH ;

Xu, CJ ;

Lin, GC .

PROCESS BIOCHEMISTRY, 2006, 41 (03) :734-738

[39] Influence of enzyme aqueous source on RME-based purification of α-amylase [J].

Tonova, K ;

Lazarova, Z .

SEPARATION AND PURIFICATION TECHNOLOGY, 2005, 47 (1-2) :43-51

[40] Reversed micelle solvents as tools of enzyme purification and enzyme-catalyzed conversion [J].

Tonova, Konstantza ;

Lazarova, Zdravka .

BIOTECHNOLOGY ADVANCES, 2008, 26 (06) :516-532

← 1 2 3 4 5 →