Quantitation of yeast ceramides using high-performance liquid chromatography-evaporative light-scattering detection

被引：18

作者：

Zhou, Q

Zhang, L

Fu, XQ

Chen, GQ ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 780卷 / 01期

关键词：

yeast cells; ceramides;

D O I：

10.1016/S1570-0232(02)00466-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC-ELSD) was used to assay the ceramides in yeast cells. The HPLC-ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：161 / 169

页数：9