Maternal separation with early weaning impairs neuron-glia integrity: non-invasive evaluation and substructure demonstration

被引:11
作者
Zeng, Haiyan [1 ,2 ]
Zhang, Xiaolei [3 ]
Wang, Wenqiang [2 ]
Shen, Zhiwei [3 ]
Dai, Zhuozhi [3 ]
Yu, Zhijia [1 ]
Xu, Shuqin [4 ]
Yan, Gen [3 ]
Huang, Qingjun [1 ]
Wu, Renhua [3 ]
Chen, Xi [5 ]
Xu, Haiyun [1 ,4 ,6 ]
机构
[1] Shantou Univ, Mental Hlth Ctr, Med Coll, Shantou, Peoples R China
[2] Xianyue Hosp, Xiamen Mental Hlth Ctr, Xiamen, Peoples R China
[3] Shantou Univ, Affiliated Hosp 2, Dept Med Imaging, Med Coll, Shantou, Peoples R China
[4] Shantou Univ, Dept Anat, Med Coll, Shantou, Peoples R China
[5] Harvard Med Sch, McLean Hosp, McLean Imaging Ctr, Belmont, MA USA
[6] Wenzhou Med Univ, Sch Psychiat, Wenzhou, Peoples R China
关键词
GLUTAMATE TRANSPORTER; N-ACETYLASPARTATE; PREFRONTAL CORTEX; MOUSE MODEL; STRESS MODELS; BRAIN; ASTROCYTES; LOCALIZATION; HOMEOSTASIS; RECEPTORS;
D O I
10.1038/s41598-020-76640-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Astrocytes and oligodendrocytes play essential roles in regulating neural signal transduction along neural circuits in CNS. The perfect coordination of neuron/astrocyte and neuron/oligodendrocyte entities was termed as neuron-glia integrity recently. Here we monitored the status of neuron-glia integrity via non-invasive neuroimaging methods and demonstrated the substructures of it using other approaches in an animal model of maternal separation with early weaning (MSEW), which mimics early life neglect and abuse in humans. Compared to controls, MSEW rats showed higher glutamate level, but lower GABA in prefrontal cortex (PFC) detected by chemical exchange saturation transfer and H-1-MRS methods, lower levels of glial glutamate transporter-1 and ATP-alpha, but increased levels of glutamate decarboxylase-65 and glutamine synthetase in PFC; reduced fractional anisotropy in various brain regions revealed by diffusion tensor imaging, along with increased levels of N-acetyl-aspartate measured by H-1-MRS; and hypomyelination in PFC as evidenced by relevant cellular and molecular changes.
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页数:14
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