TRANSCRIPTOMIC ANALYSIS REVEALS DIFFERENTIAL ACTIVATION OF MICROGLIAL GENES AFTER ISCHEMIC STROKE IN MICE

被引：18

作者：

Khan, Akbar ^{[1
,2
]}

Ju, Furong ^{[1
]}

Xie, Wenguang ^{[1
]}

Hafeez, Muhammad Tariq ^{[1
]}

Cheng, Xiaofeng ^{[1
]}

Yang, Zhijie ^{[1
]}

Zhu, Lirui ^{[1
]}

Li, Ting ^{[1
]}

Zhang, Shengxiang ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou 730000, Peoples R China

[2] Karakoram Int Univ, Dept Biol Sci, Gilgit Baltistan, Pakistan

来源：

NEUROSCIENCE | 2017年 / 348卷

基金：

中国国家自然科学基金;

关键词：

transcriptome; digital gene expression; stroke; microglia; activation; photothrombosis; CHEMOKINE RECEPTORS; REACTIVE MICROGLIA; NERVOUS-SYSTEM; BRAIN-INJURY; C-JUN; CELLS; ADULT; EXPRESSION; PROLIFERATION; INFLAMMATION;

D O I：

10.1016/j.neuroscience.2017.02.019

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Microglia are immune cells in the brain and play a pivotal role in the progression of ischemic injury, but the gene expression and signaling pathways related to the activation of microglia following ischemia remain unclear. In our experiment, we used digital gene expression (DGE) analysis to profile the transcriptome of ischemic tissue in a photothrombosis model. DGE analysis identified that a total of 749 genes were differentially regulated (643 up-regulated and 106 down-regulated) after 2 days and 7 days following stroke. We found 74.5% of these differentially expressed genes were microglial genes. Gene ontology (GO) analysis categorizes these differentially expressed genes at 2 days and 7 days to specific biological processes such as inflammatory response, cell activation, cell proliferation, and chemokine and cytokine production. Our data demonstrated that a large number of microglial genes were highly regulated at 2 days after stroke, but the number of differentially expressed genes had reduced drastically by 7 days. Importantly, some of the differentially expressed microglial genes at 7 days did not show differential expression at 2 days after stroke. DGE analysis indicated that specific genes related to microgliosis were regulated after ischemia. Consistent with the changes in transcriptome, the results from histological analysis of transgenic mice revealed that the microglia proliferated and aggregated surrounding the ischemic core during the period from 2 days to 7 days following photothrombosis. Together, these results suggested that transcriptomic changes in microglial genes after stroke may have a profound implication for pathophysiology and treatment of stroke. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：212 / 227

页数：16

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