Optimization of polysaccharide and ergosterol production from Agaricus brasiliensis by fermentation process

被引：42

作者：

Gao, Hong ^{[1
]}

Gu, Wen-Ying ^{[1
]}

机构：

[1] So Yangtze Univ, Sch Food Sci & Technol, Wuxi 214036, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2007年 / 33卷 / 03期

关键词：

Agaricus brasiliensis; ergosterol; experimental designs; fermentation; optimization; polysaccharide;

D O I：

10.1016/j.bej.2006.10.022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Statistically based experimental designs were applied for the fermentation optimization of polysaccharide and ergosterol production from Agaricus brasiliensis in a stirred-fermenter. Culture temperature, agitation speed and initial pH were identified to have significant effects on polysaccharide and ergosterol production by a Plackett-Burman design. These three significant factors were subsequently optimized using steepest ascent method and Box-Behnken design. The validity of the optimum conditions was verified by separate experiments in which the polysaccharide and ergosterol yields were increased by 57% and 43%, respectively, as compared to those under unoptimized fermentation conditions. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：202 / 210

页数：9

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