Optimization of polysaccharide and ergosterol production from Agaricus brasiliensis by fermentation process

被引:42
作者
Gao, Hong [1 ]
Gu, Wen-Ying [1 ]
机构
[1] So Yangtze Univ, Sch Food Sci & Technol, Wuxi 214036, Peoples R China
关键词
Agaricus brasiliensis; ergosterol; experimental designs; fermentation; optimization; polysaccharide;
D O I
10.1016/j.bej.2006.10.022
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Statistically based experimental designs were applied for the fermentation optimization of polysaccharide and ergosterol production from Agaricus brasiliensis in a stirred-fermenter. Culture temperature, agitation speed and initial pH were identified to have significant effects on polysaccharide and ergosterol production by a Plackett-Burman design. These three significant factors were subsequently optimized using steepest ascent method and Box-Behnken design. The validity of the optimum conditions was verified by separate experiments in which the polysaccharide and ergosterol yields were increased by 57% and 43%, respectively, as compared to those under unoptimized fermentation conditions. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:202 / 210
页数:9
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