Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001

被引：29
作者：
Miagostovich, MP [1 ]
dos Santos, FB [1 ]
de Simone, TS [1 ]
Costa, EV [1 ]
Filippis, AMB [1 ]
Schatzmayr, HG [1 ]
Nogueira, RMR [1 ]
机构：
[1] Fiocruz MS, Inst Oswaldo Cruz, Fdn Oswaldo Cruz,Pavilhao Cardoso Fontes, Dept Virol, BR-21045900 Rio De Janeiro, RJ, Brazil
来源：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2002年 / 35卷 / 08期
关键词：
dengue viruses type 3; sequencing; RSS-PCR; Rio de Janeiro; Brazil;
D O I：
10.1590/S0100-879X2002000800002
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.
引用
收藏
页码：869 / 872
页数：4
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