Cultivation of human fibroblasts and multipotent mesenchymal stromal cells on mesoporous silica and mixed metal oxide films

被引：10

作者：

Boecking, D. ^{[1
]}

Fiedler, J. ^{[2
]}

Brenner, R. E. ^{[2
]}

Huesing, N. ^{[1
]}

机构：

[1] Univ Ulm, Inst Inorgan Chem 1, D-89081 Ulm, Germany

[2] Univ Ulm, Div Biochem Joint & Connect Tissue Dis, Dept Orthopaed, D-89081 Ulm, Germany

来源：

JOURNAL OF MATERIALS SCIENCE | 2009年 / 44卷 / 24期

关键词：

TIO2-SIO2 IMPLANT COATINGS; DIRECT TISSUE ATTACHMENT; GEL; NANOSTRUCTURES;

D O I：

10.1007/s10853-009-3565-x

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The application of mesoporous silica and silica-titania-mixed metal oxide films prepared via sol-gel processing as substrates for cell growth was investigated. A deliberate tailoring of the chemical composition of the porous substrates with different Si:Ti ratios was achieved by using a single-source precursor based on a titanium-coordinated alkoxysilane, resulting in mesoporous silica-titania films with hydrophilic surfaces. The different coatings were investigated with respect to their applicability in the cultivation of human cells such as human fibroblasts and multipotent mesenchymal stromal cells. It was found that they promoted cell adhesion and proliferation of human fibroblasts up to a period of 14 days. After 2 weeks only single apoptotic cells could be detected on silica-titania mixed oxide films in contrast to a somewhat higher amount on silica coatings. Furthermore, none of the films inhibited osteogenic differentiation of multipotent mesenchymal stromal cells.

引用

页码：6786 / 6794

页数：9

共 36 条

[1] Sol-gel-derived TiO2-SiO2 implant coatings for direct tissue attachment.: Part I:: design, preparation and characterization [J].