Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

被引:6
作者
Sarto, Marcella Paula Mansano [1 ]
Lucas da Silva, Hevillyn Fernanda [1 ]
de Souza Fernandes, Nilma [2 ]
de Abreu, Ana Paula [1 ]
Zanusso Junior, Gerson [1 ]
de Ornelas Toledo, Max Jean [1 ,2 ,3 ]
机构
[1] Univ Estadual Maringa, Hlth Sci Ctr, Postgrad Program Hlth Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Biol Sci Ctr, Postgrad Program Biol Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Hlth Sci Ctr, Dept Basic Hlth Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
关键词
Trypanosoma cruzi; Mice; Syzygium aromaticum; Zingiber officinale; Drug combination; Oral Chagas disease; CHAGAS-DISEASE; IN-VITRO; INFECTION; CHEMOTHERAPY; EFFICACY; BLOOD;
D O I
10.1186/s12906-021-03248-8
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background Trypanosoma cruzi is the etiological agent of Chagas disease (CD) or American trypanosomiasis, an important public health problem in Latin America. Benznidazole (BZ), a drug available for its treatment, has limited efficacy and significant side effects. Essential oils (EOs) have demonstrated trypanocidal activity and may constitute a therapeutic alternative. Our aim was to evaluate the efficacy of the EOs of clove (CEO - Syzygium aromaticum) and ginger (GEO - Zingiber officinale), administered alone and in combination with BZ, in Swiss mice infected with T. cruzi. Methods The animals were inoculated with 10,000 blood trypomastigotes of the Y strain of T. cruzi II by gavage and divided into four groups (n = 12 to 15): 1) untreated control (NT); 2) treated with BZ; 3) treated with CEO or GEO; and 4) treated with BZ + CEO or GEO. The treatments consisted of oral administration of 100 mg/kg/day, from the 5th day after parasite inoculation, for 20 consecutive days. All groups were submitted to fresh blood examination (FBE), blood culture (BC), conventional PCR (cPCR) and real-time PCR (qPCR), before and after immunosuppression with cyclophosphamide. Results Clove and ginger EOs, administered alone and in combination with BZ, promoted suppression of parasitemia (p < 0.0001), except for the animals treated with CEO alone, which presented a parasitemia curve similar to NT animals. However, there was a decrease in the BC positivity rate (p < 0.05) and parasite load (< 0.0001) in this group. Treatment with GEO alone, on the other hand, besides promoting a decrease in the BC positivity rate (p < 0.05) and parasite load (p < 0.01), this EO also resulted in a decrease in mortality rate (p < 0.05) of treated mice. Conclusions Decreased parasite load, as detected by qPCR, was observed in all treatment groups (BZ, CEO, GEO and BZ + EOs), demonstrating benefits even in the absence of parasitological cure, thus opening perspectives for further studies.
引用
收藏
页数:11
相关论文
共 39 条
[1]   Essential oils: in vitro activity against Leishmania amazonensis, cytotoxicity and chemical composition [J].
Andrade, Milene Aparecida ;
Azevedo, Clenia dos Santos ;
Motta, Flavia Nader ;
dos Santos, Maria Lucilia ;
Silva, Camila Lasse ;
de Santana, Jaime Martins ;
Bastos, Izabela M. D. .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2016, 16
[2]   SPECIFIC CHEMOTHERAPY OF CHAGAS-DISEASE - A COMPARISON BETWEEN THE RESPONSE IN PATIENTS AND EXPERIMENTAL-ANIMALS INOCULATED WITH THE SAME STRAINS [J].
ANDRADE, SG ;
RASSI, A ;
MAGALHAES, JB ;
FILHO, FF ;
LUQUETTI, AO .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1992, 86 (06) :624-626
[3]   Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth [J].
Azeredo, Camila M. O. ;
Soares, Maurilio J. .
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, 2013, 23 (05) :762-768
[4]   In vitro biological evaluation of eight different essential oils against Trypanosoma cruzi, with emphasis on Cinnamomum verum essential oil [J].
Azeredo, Camila Maria O. ;
Santos, Thalita Gilda ;
de Noronha Sales Maia, Beatriz Helena Lameiro ;
Soares, Maurilio Jose .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2014, 14
[5]   Therapeutical approaches under investigation for treatment of Chagas disease [J].
Bahia, Maria Terezinha ;
Diniz, Livia de Figueiredo ;
Furtado Mosqueira, Vanessa Carla .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2014, 23 (09) :1225-1237
[6]   Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection? [J].
Barreto-de-Albuquerque, Juliana ;
Silva-dos-Santos, Danielle ;
Perez, Ana Rosa ;
Berbert, Luiz Ricardo ;
de Santana-van-Vliet, Eliane ;
Farias-de-Oliveira, Desio Aurelio ;
Moreira, Otacilio C. ;
Roggero, Eduardo ;
de Carvalho-Pinto, Carla Eponina ;
Jurberg, Jose ;
Cotta-de-Almeida, Vincius ;
Bottasso, Oscar ;
Savino, Wilson ;
de Meis, Juliana .
PLOS NEGLECTED TROPICAL DISEASES, 2015, 9 (06)
[7]   The oral transmission of Chagas' disease: An acute form of infection responsible for regional outbreaks [J].
Benchimol Barbosa, Paulo Roberto .
INTERNATIONAL JOURNAL OF CARDIOLOGY, 2006, 112 (01) :132-133
[8]   Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts [J].
Bértoli, M ;
Andó, M ;
Toledo, MJD ;
De Araújo, SM ;
Gomes, ML .
PARASITOLOGY RESEARCH, 2006, 99 (01) :7-13
[9]  
Braga M. Socorro, 2000, Revista do Instituto de Medicina Tropical de Sao Paulo, V42, P157, DOI 10.1590/S0036-46652000000300009
[10]  
Brasil MS, 2019, B EPIDEMIOL, V50, P10