A resazurin-based biosensor for organic pollutants

被引：42

作者：

Tizzard, Aynsley C.

Bergsma, Joost H.

Lloyd-Jones, Gareth

机构：

[1] Landcare Res, Lincoln 8152, New Zealand

[2] Lincoln Univ, Lincoln Ventures, Lincoln 8152, New Zealand

来源：

BIOSENSORS & BIOELECTRONICS | 2006年 / 22卷 / 05期

关键词：

resazurin; toluene; BTEX; Pseudomonas; Burkholderia; redox-dye;

D O I：

10.1016/j.bios.2006.01.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new rapid biosensor method employing the dye resazurin as an indicator of bacterial respiration has been developed to provide a rapid, facile and specific biosensor for environmental contaminants that does not rely on genetic modification techniques, is suitable for a high-throughput multiwell format, and is ideally suited to resource-cons trained environmental monitoring situations. This whole-cell biosensor has been applied to the test analyte toluene using natural toluene-degrading bacteria as the biological component and is competitive with more complex recombinant approaches. The redox-driven biosensor is dependent on the catabolism of a specific compound, concomitantly reducing the redox indicator resazurin to provide the analytical signal in a whole-cell biosensor assay. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：759 / 763

页数：5

共 35 条

[1] EVALUATION OF ALAMAR COLORIMETRIC MIC METHOD FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF GRAM-NEGATIVE BACTERIA [J].