A Quick HYL1-Dependent Reactivation of MicroRNA Production Is Required for a Proper Developmental Response after Extended Periods of Light Deprivation

被引:55
作者
Achkar, Natalia P. [1 ]
Cho, Seok Keun [2 ]
Poulsen, Christian [4 ]
Arce, Agustin L. [1 ]
Re, Delfina A. [1 ]
Giudicatti, Axel J. [1 ]
Karayekov, Elizabeth [3 ]
Ryu, Moon Young [2 ]
Choi, Suk Won [2 ]
Harholt, Jesper [4 ]
Casal, Jorge J. [3 ,5 ]
Yang, Seong Wook [2 ,6 ]
Manavella, Pablo A. [1 ]
机构
[1] CONICET UNL FBCB, Inst Agrobiotecnol Litoral, RA-3000 Santa Fe, Argentina
[2] Yonsei Univ, Dept Syst Biol, Coll Life Sci & Biotechnol, Seoul, South Korea
[3] Univ Buenos Aires, Fac Agron, IFEVA, RA-1417 Buenos Aires, DF, Argentina
[4] Carlsberg Res Lab, DK-1799 Copenhagen V, Denmark
[5] Consejo Nacl Invest Cient & Tecn, Fdn Inst Leloir, Inst Invest Bioquim Buenos Aires, RA-1405 Buenos Aires, DF, Argentina
[6] Univ Copenhagen, Dept Plant & Environm Sci, Fac Sci, Copenhagen, Denmark
基金
新加坡国家研究基金会;
关键词
MIRNA BIOGENESIS; RNA BIOGENESIS; PRI-MIRNA; HYL1; PHOSPHORYLATION;
D O I
10.1016/j.devcel.2018.06.014
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Light is the most influential environmental stimulus for plant growth. In response to deficient light, plants reprogram their development to adjust their growth in search for a light source. A fine reprogramming of gene expression orchestrates this adaptive trait. Here we show that plants alter microRNA (miRNA) biogenesis in response to light transition. When plants suffer an unusual extended period of light deprivation, the miRNA biogenesis factor HYPONASTIC LEAVES 1 (HYL1) is degraded but an inactive pool of phosphorylated protein remains stable inside the nucleus. Degradation of HYL1 leads to the release of gene silencing, triggering a proper response to dark and shade. Upon light restoration, a quick dephosphorylation of HYL1 leads to the reactivation of miRNA biogenesis and a switch toward a developmental program that maximizes the light uptake. Our findings define a unique and fast regulatory mechanism controlling the plant silencing machinery during plant light response.
引用
收藏
页码:236 / +
页数:18
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