A disposable electrochemical immunofiltration test strip for rapid detection of α-fetoprotein

被引：16

作者：

Chen, Zhencheng ^{[1
]}

Fang, Cheng ^{[1
]}

Wang, Hongyan ^{[1
]}

He, Jishan ^{[1
]}

Deng, Zhensheng ^{[1
]}

机构：

[1] Cent S Univ, Dept Biomed Engn, Sch Infophys & Geomat Engn, Changsha 410083, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 141卷 / 02期

关键词：

alpha-Fetoprotein (AFP); Immunofiltration; Test strip; Ferrocene monocarboxylic acid; HEPATOCELLULAR-CARCINOMA; TUMOR-MARKERS; IMMUNOASSAY; ASSAY; SYSTEM; LABEL; IMMUNOSENSORS; BIOSENSOR;

D O I：

10.1016/j.snb.2009.07.029

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A disposable electrochemical immunofiltration test strip for the rapid detection of alpha-fetoprotein (AFP) was developed. The test strip was constructed by assembly of screen-printed carbon electrodes, absorption-water pad, nitrocellulose membrane modified by anti-AFP antibody and glass fiber membrane conjugated with ferrocene monocarboxylic acid (FC) labeling AFP. The analytical system utilizes flow-through immunofiltration and competitive immunoassay techniques in combination with an amperometric sensor. The parameters affecting the immunoassay such as selection of filter membrane, membrane pore-size, and antibody binding capacity were investigated and optimized. The immunofiltration system allows us to specifically and directly detect AFP in serum with a low detection limit of 6 ng/mL The working range is from 6 to 500 ng/mL with an overall analysis time of 5 min for one sample. This electrochemical immunoassay system enabled us to construct a novel point-of-care testing device for the monitoring of biomarker including AFP. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：436 / 440

页数：5

共 31 条

[1] Immunobiology of the hepatocellular carcinoma associated tumor antigen alpha-fetoprotein [J].

Ali, M ;

Ritter, M ;

Grimm, CF ;

Boecher, WO ;

Blum, HE ;

Geissler, M .

JOURNAL OF HEPATOLOGY, 2003, 38 :88-88

[2] CHEMILUMINESCENT ENZYME-IMMUNOASSAY FOR ALPHA-FETOPROTEIN USING BETA-D-GALACTOSIDASE AS LABEL AND 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE AS SUBSTRATE [J].

ARAKAWA, H ;

IKEGAMI, T ;

MAEDA, M ;

TSUJI, A .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1993, 8 (03) :135-138

[3] Alpha-fetoprotein in the early neonatal period - a large study and review of the literature [J].

Bader, D ;

Riskin, A ;

Vafsi, O ;

Tamir, A ;

Peskin, B ;

Israel, N ;

Merksamer, R ;

Dar, H ;

David, M .

CLINICA CHIMICA ACTA, 2004, 349 (1-2) :15-23

[4] ALPHA-FETOPROTEIN LEVELS IN NORMAL ADULTS [J].

BALL, D ;

ROSE, E ;

ALPERT, E .

AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1992, 303 (03) :157-159

[5] DETECTION OF STAPHYLOCOCCUS-AUREUS WITH BIOTINYLATED MONOCLONAL-ANTIBODIES DIRECTED AGAINST STAPHYLOCOCCAL TNASE COMPLEXED TO AVIDIN-PEROXIDASE IN A RAPID SANDWICH ENZYME-LINKED IMMUNOFILTRATION ASSAY (SELIFA) [J].

BRAKSTAD, OG ;

MAELAND, JA .

JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 39 (02) :128-134

[6] Diagnosing and monitoring hepatocellular carcinoma with alpha-fetoprotein: New aspects and applications [J].

Debruyne, Evi N. ;

Delanghe, Joris R. .

CLINICA CHIMICA ACTA, 2008, 395 (1-2) :19-26

[7]

Frew J. E., 1987, ANAL CHEM, V59, p933A

[8] Prussian blue modified amperometric FIA biosensor:: one-step immunoassay for α-fetoprotein [J].

Guan, JG ;

Miao, YQ ;

Chen, JR .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (08) :789-794

[9] High efficiency electrochemical immuno sensors using 3D comb electrodes [J].

Honda, N ;

Inaba, M ;

Katagiri, T ;

Shoji, S ;

Sato, H ;

Homma, T ;

Osaka, T ;

Saito, M ;

Mizuno, J ;

Wada, Y .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (11) :2306-2309

[10] OPTIMIZING THE SOLID-PHASE IMMUNOFILTRATION ASSAY - A RAPID ALTERNATIVE TO IMMUNOASSAYS [J].

IJSSELMUIDEN, OE ;

HERBRINK, P ;

MEDDENS, MJM ;

TANK, B ;

STOLZ, E ;

VANEIJK, RVW .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 119 (01) :35-43

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