Increased production of bacterial cellulose by Acetobacter sp V6 in synthetic media under shaking culture conditions

被引：123

作者：

Son, HJ ^{[1
]}

Kim, HG

Kim, KK

Kim, HS

Kim, YG

Lee, SJ

机构：

[1] Miryang Natl Univ, Dept Biotechnol, Miryang, Kyungnam, South Korea

[2] Pusan Natl Univ, Dept Microbiol, Pusan 609735, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2003年 / 86卷 / 03期

关键词：

Acetobacter sp; bacterial cellulose; cosubstrate; synthetic media;

D O I：

10.1016/S0960-8524(02)00176-1

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Acetobacter strains are bacteria that can synthesize cellulose when grown in a complex medium containing glucose. The effect of the components of a synthetic medium on bacterial cellulose (BC) production by a newly isolated Acetobacter sp. V6 in shaking cultures was investigated. BC production was dependent on the presence of MgSO4 . 7H(2)O and cosubstrates such as ethanol and lactic acid in the medium. The optimal synthetic medium contained 1.5% glucose, 0.2% (NH4)(2)SO4, 0.3% KH2PO4, 0.3% Na2HPO4 . 12H(2)O, 0.08% MgSO4 . 7H(2)O, 0.0005% FeSO4 .7H(2)O, 0.0003% H3BO3, 0.00005% nicotinamide, and 0.6% ethanol. A maximum BC concentration of 4.16 g/l was achieved after 8 days of cultivation at 200 rpm. The production of BC by Acetobacter sp. V6 was higher in synthetic medium than complex medium (Hestrin and Schramm medium) traditionally used for Acetobacter strains. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：215 / 219

页数：5

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