Computer aided-molecular design and synthesis of a high selective molecularly imprinted polymer for solid-phase extraction of furosemide from human plasma

被引:97
作者
Gholivand, Mohammad Bagher [1 ]
Khodadadian, Mehdi [1 ]
Ahmadi, Farhad [2 ]
机构
[1] Razi Univ, Fac Sci, Dept Chem, Kermanshah, Iran
[2] Kermanshah Univ Med Sci, Fac Pharm, Dept Pharmaceut Chem, Kermanshah, Iran
关键词
Molecular imprinted polymer (MIP); Ab initio calculation; Furosemide; Separation; PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; COMPUTATIONAL DESIGN; HUMAN URINE; SOLVENT; ASSAY; ACID; DISTRIBUTIONS; EQUILIBRIA; DIURETICS;
D O I
10.1016/j.aca.2009.11.019
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Highly selective molecularly imprinted polymers (MIPs) for solid-phase extraction and determination of furosemide in human plasma have been designed and prepared. In order to study the intermolecular interactions in the pre-polymerization mixture and to find a suitable functional monomer in MIP preparation, a computational approach was developed. It was based on the comparison of the binding energy of the complexes between the template and functional monomers. Having confirmed the results of computational method. three MIPs were synthesized with different functional monomers, i.e. acrylamide (AAM), 4-vinylpiridine (4-VP) and acrylonitrile (ACN), and then evaluated using Langmuir-Freundlich (LF) isotherm. Using the MIP prepared by AAM as functional monomer, a molecularly imprinted solid-phase extraction procedure followed by high performance liquid chromatography with ultraviolet detector (MISPE-HPLC-UV) was developed for selective extraction and determination of furosemide in human plasma. For the proposed MISPE-HPLC-UV method, the linearity between responses (peak area) and concentration was found over the range of 75-3500 ng mL(-1) with a linear regression coefficient (R-2) of 0.997. The limit of detection (LOD) and quantification (LOQ) in plasma were 12.9 and 43.3 ng mL(-1), respectively. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:225 / 232
页数:8
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