Isolation and characterization of microsatellite loci from the forage species Arachis pintoi (Genus Arachis)

被引：25

作者：

Palmieri, DA ^{[1
]}

Hoshino, AA ^{[1
]}

Bravo, JP ^{[1
]}

Lopes, CR ^{[1
]}

Gimenes, MA ^{[1
]}

机构：

[1] Univ Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期

关键词：

microsatellite markers; genetic variation; Arachis pintoi;

D O I：

10.1046/j.1471-8286.2002.00317.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arachis pintoi is an alternative to forage production in the tropics. Its germplasm comprises more than 150 accessions, that could be used to improve it. Our objective was the isolation and characterization of microsatellite loci in A. pintoi to be used to molecular evaluation of this germplasm and of A. repens (section Caulorrhizae). Seven loci were analyzed using five accessions of A. repens and 20 accessions of A. pintoi. The high variation found makes clear the high potential of this marker in genetic studies in these species. The developed markers showed total transferability to A. repens.

引用

页码：551 / 553

页数：3

共 8 条

[1]

[Anonymous], 1998, Primer 3

[2] Genetic diversity among European cultivated spelt revealed by microsatellites [J].

Bertin, P ;

Grégoire, D ;

Massart, S ;

de Froidmont, D .

THEORETICAL AND APPLIED GENETICS, 2001, 102 (01) :148-156

[3] Development and characterization of microsatellite markers in Cucumis [J].

Danin-Poleg, Y ;

Reis, N ;

Tzuri, G ;

Katzir, N .

THEORETICAL AND APPLIED GENETICS, 2001, 102 (01) :61-72

[4]

Ferreira M.E., 1996, INTRO AO USO MARCADO, V2

[5] Discovery and characterization of polymorphic simple sequence repeats (SSRs) in peanut [J].

Hopkins, MS ;

Casa, AM ;

Wang, T ;

Mitchell, SE ;

Dean, RE ;

Kochert, GD ;

Kresovich, S .

CROP SCIENCE, 1999, 39 (04) :1243-1247

[6]

KIJAS JMH, 1994, BIOTECHNIQUES, V16, P656

[7] Diversity, genetic structure and evidence of outcrossing in British populations of the rock fern Adiantum capillus-veneris using microsatellites [J].

Pryor, KV ;

Young, JE ;

Rumsey, FJ ;

Edwards, KJ ;

Bruford, MW ;

Rogers, HJ .

MOLECULAR ECOLOGY, 2001, 10 (08) :1881-1894

[8] Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system [J].

Senior, ML ;

Murphy, JP ;

Goodman, MM ;

Stuber, CW .

CROP SCIENCE, 1998, 38 (04) :1088-1098

← 1 →