Leucyl-tRNA Synthetase-dependent and -independent Activation of a Group I Intron

被引：10

作者：

Boniecki, Michal T. ^{[1
]}

Rho, Seung Bae ^{[1
]}

Tukalo, Mikhail ^{[1
]}

Hsu, Jennifer L. ^{[1
]}

Romero, Eliana P. ^{[1
]}

Martinis, Susan A. ^{[1
]}

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 39期

基金：

美国国家卫生研究院;

关键词：

YEAST MITOCHONDRIA; MATURASE DEFICIENCY; SPLICING FUNCTION; DNA ENDONUCLEASE; CATALYTIC CORE; P4-P6; DOMAIN; PROTEIN; BINDING; CONNECTIONS; MUTATIONS;

D O I：

10.1074/jbc.M109.031179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leucyl-tRNA synthetase (LeuRS) is an essential RNA splicing factor for yeast mitochondrial introns. Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron. RNA deletion mutants of the large bI4 intron were constructed to identify a competently folded intron for biochemical analysis. The minimized bI4 intron was active in RNA splicing and contrasts with previous proposals that the canonical core of the bI4 intron is deficient for catalysis. The activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.

引用

页码：26243 / 26250

页数：8

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