Leucyl-tRNA Synthetase-dependent and -independent Activation of a Group I Intron

被引：10

作者：

Boniecki, Michal T. ^{[1
]}

Rho, Seung Bae ^{[1
]}

Tukalo, Mikhail ^{[1
]}

Hsu, Jennifer L. ^{[1
]}

Romero, Eliana P. ^{[1
]}

Martinis, Susan A. ^{[1
]}

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 39期

基金：

美国国家卫生研究院;

关键词：

YEAST MITOCHONDRIA; MATURASE DEFICIENCY; SPLICING FUNCTION; DNA ENDONUCLEASE; CATALYTIC CORE; P4-P6; DOMAIN; PROTEIN; BINDING; CONNECTIONS; MUTATIONS;

D O I：

10.1074/jbc.M109.031179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leucyl-tRNA synthetase (LeuRS) is an essential RNA splicing factor for yeast mitochondrial introns. Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron. RNA deletion mutants of the large bI4 intron were constructed to identify a competently folded intron for biochemical analysis. The minimized bI4 intron was active in RNA splicing and contrasts with previous proposals that the canonical core of the bI4 intron is deficient for catalysis. The activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.

引用

页码：26243 / 26250

页数：8

共 36 条

[1] A PROTEIN REQUIRED FOR SPLICING GROUP-I INTRONS IN NEUROSPORA MITOCHONDRIA IS MITOCHONDRIAL TYROSYL-TRANSFER RNA-SYNTHETASE OR A DERIVATIVE THEREOF [J].

AKINS, RA ;

LAMBOWITZ, AM .

CELL, 1987, 50 (03) :331-345

[2] Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA [J].

Bassi, GS ;

de Oliveira, DM ;

White, MF ;

Weeks, KM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) :128-133

[3] A tyrosyl-tRNA synthetase protein induces tertiary folding of the group I intron catalytic core [J].

Caprara, MG ;

Mohr, G ;

Lambowitz, AM .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 257 (03) :512-531

[4] A tyrosyl-tRNA synthetase recognizes a conserved tRNA-like structural motif in the group I intron catalytic core [J].

Caprara, MG ;

Lehnert, V ;

Lambowitz, AM ;

Westhof, E .

CELL, 1996, 87 (06) :1135-1145

[5] Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) with the group I intron P4-P6 domain.: Thermodynamic analysis and the role of metal ions [J].

Caprara, MG ;

Myers, CA ;

Lambowitz, AM .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 308 (02) :165-190

[6] Function of tyrosyl-tRNA synthetase in splicing group I introns: An induced-fit model for binding to the P4-P6 domain based on analysis of mutations at the junction of the P4-P6 stacked helices [J].

Chen, X ;

Gutell, RR ;

Lambowitz, AM .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 301 (02) :265-283

[7] FUNCTION OF NEUROSPORA MITOCHONDRIAL TYROSYL-TRANSFER RNA-SYNTHETASE IN RNA SPLICING REQUIRES AN IDIOSYNCRATIC DOMAIN NOT FOUND IN OTHER SYNTHETASES [J].

CHERNIACK, AD ;

GARRIGA, G ;

KITTLE, JD ;

AKINS, RA ;

LAMBOWITZ, AM .

CELL, 1990, 62 (04) :745-755

[8] SITE-SPECIFIC DNA ENDONUCLEASE AND RNA MATURASE ACTIVITIES OF 2 HOMOLOGOUS INTRON-ENCODED PROTEINS FROM YEAST MITOCHONDRIA [J].

DELAHODDE, A ;

GOGUEL, V ;

BECAM, AM ;

CREUSOT, F ;

PEREA, J ;

BANROQUES, J ;

JACQ, C .

CELL, 1989, 56 (03) :431-441

[9] INVITRO SPLICING OF THE TERMINAL INTERVENING SEQUENCE OF SACCHAROMYCES-CEREVISIAE CYTOCHROME-B PRE-MESSENGER-RNA [J].

GAMPEL, A ;

TZAGOLOFF, A .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (07) :2545-2551

[10] CONNECTIONS BETWEEN RNA SPLICING AND DNA INTRON MOBILITY IN YEAST MITOCHONDRIA - RNA MATURASE AND DNA ENDONUCLEASE SWITCHING EXPERIMENTS [J].

GOGUEL, V ;

DELAHODDE, A ;

JACQ, C .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (02) :696-705

← 1 2 3 4 →