Room-Temperature Infrared Spectroscopy Combined with Mass Spectrometry Distinguishes Gas-Phase Protein Isomers

被引：26
作者：
Fung, Y. M. Eva [1 ]
Besson, Thierry [2 ]
Lemaire, Joel [2 ]
Maitre, Philippe [2 ]
Zubarev, Roman A. [1 ]
机构：
[1] Karolinska Inst, Inst Med Biochem & Biophys, Div Mol Biometry, S-17177 Stockholm, Sweden
[2] Univ Paris 11, CNRS, Chim Phys Lab, F-91405 Orsay, France
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 44期
关键词：
IR spectroscopy; mass spectrometry; proteins; protein structures; ELECTRON-CAPTURE DISSOCIATION; IONS; PEPTIDES; CATIONS;
D O I：
10.1002/anie.200901516
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Differential IR spectra of gas-phase proteins at room temperature can be obtained by a combination of IR multiphoton dissociation (IRMPD) and FT ion cyclotron resonance mass spectrometry. This new approach yields unique spectra for all-L and singly D-substituted isomers of the protein Trp-cage (see depicted differential spectrum) and thus avoids possible unfolding of proteins during IR irradiation that can affect conventional IRMPD measurements. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：8340 / 8342
页数：3
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