Development of a rapid antiviral screening assay based on eGFP reporter virus of Mayaro virus

被引：14
作者：
Li, Xiaodan [1 ,2 ]
Zhang, Hongqing [1 ]
Zhang, Yanan [1 ]
Li, Jiaqi [1 ]
Wang, Zhen [1 ]
Deng, Chenglin [1 ]
Gomes Jardim, Ana Carolina [3 ]
Terzian, Ana Carolina B. [4 ]
Nogueira, Mauricio Lacerda [4 ]
Zhang, Bo [1 ]
机构：
[1] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China
[2] Hunan Normal Univ, Sch Med, Changsha 410081, Hunan, Peoples R China
[3] Univ Fed Uberlandia, Inst Biomed Sci, Lab Virol, Uberlandia, MG, Brazil
[4] Sao Jose Do Rio Preto Sch Med FAMERP, Sao Jose Do Rio Preto, SP, Brazil
来源：
ANTIVIRAL RESEARCH | 2019年 / 168卷
基金：
中国国家自然科学基金;
关键词：
Mayaro virus; Reverse genetics; Reporter virus; Antiviral drug discovery; CHIKUNGUNYA; REPLICATION; INHIBITION; EXPRESSION; ENTRY; INFECTION; RIBAVIRIN; INSERTION; PROTEINS; OUTBREAK;
D O I：
10.1016/j.antiviral.2019.05.013
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Mayaro virus (MAYV) is a neglected mosquito-borne alphavirus that causes illness similar to Chikungunya (CHIKV), Dengue (DENY) and Zika virus (ZIKV). Currently, there is no specific treatment or vaccine against MAYV infection. To develop an efficient antiviral screening assay for MAYV, we constructed the infectious clones of MAYV strain BeAr 20290 and its eGFP reporter virus. The reporter virus exhibited high replication capacity indistinguishable with the wild type MAYV, and was genetically stable within at least five rounds of passages in BHK-21 cell. The expression of eGFP correlated well with the viral replication. Using the known inhibitor ribavirin, we confirmed that the MAYV-eGFP reporter virus could be used for antiviral screening to identify the specific inhibitors against MAYV. Using the MAYV-eGFP based antiviral assay, we found that the compound 6-Azauridine which had antiviral activity against CHIKV and SFV, showed a significant inhibitory effect on MAYV replication.
引用
收藏
页码：82 / 90
页数：9
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